Details zur Publikation

Referenztyp Zeitschriften
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Titel (primär) Identification of glycoproteins isolated from extracellular polymeric substances of full-scale anammox granular sludge
Autor Boleij, M.; Pabst, M.; Neu, T.R.; van Loosdrecht, M.C.M.; Lin, Y.;
Journal / Serie Environmental Science & Technology
Erscheinungsjahr 2018
Department FLOEK;
Band/Volume 52
Heft 22
Sprache englisch;
POF III (gesamt) T32;
Supplements https://pubs.acs.org/doi/suppl/10.1021/acs.est.8b03180/suppl_file/es8b03180_si_001.xlsx
https://pubs.acs.org/doi/suppl/10.1021/acs.est.8b03180/suppl_file/es8b03180_si_002.pdf
Abstract ANaerobic AMMonium OXidation (anammox) is an established process for efficient nitrogen removal from wastewater, relying on anammox bacteria to form stable biofilms or granules. To understand the formation, structure, and stability of anammox granules, it is important to determine the composition of the extracellular polymeric substances (EPS). The aim of this research was to elucidate the nature of the proteins, which are the major fraction of the EPS and were suspected to be glycosylated. EPS were extracted from full-scale anammox granular sludge, dominated by “Candidatus Brocadia”, and subjected to denaturing polyacrylamide gel electrophoresis. By further analysis with mass spectrometry, a high abundant glycoprotein, carrying a heterogeneous O-glycan structure, was identified. The potential glycosylation sequence motif was identical to that proposed for the surface layer protein of “Candidatus Kuenenia stuttgartiensis”. The heavily glycosylated protein forms a large fraction of the EPS and was also located by lectin staining. Therefore, we hypothesize an important role of glycoproteins in the structuring of anammox granules, comparable to the importance of glycans in the extracellular matrix of multicellular organisms. Furthermore, different glycoconjugates may have distinct roles in the matrix of granular sludge, which requires more in-depth characterization of different glycoconjugates in future EPS studies.
ID 21222
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21222
Boleij, M., Pabst, M., Neu, T.R., van Loosdrecht, M.C.M., Lin, Y. (2018):
Identification of glycoproteins isolated from extracellular polymeric substances of full-scale anammox granular sludge
Environ. Sci. Technol. 52 (22), 13127 - 13135