Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Novel approach using substrate-mediated radiolabelling of RNA to link metabolic function with the structure of microbial communities
Autor Nikolausz, M.; Palatinszky, M.; Rusznyák, A.; Richnow, H.H.; Kappelmeyer, U.; Kästner, M.;
Journal / Serie FEMS Microbiology Letters
Erscheinungsjahr 2007
Department ISOBIO; UBT;
Band/Volume 274
Heft 1
Sprache englisch;
Abstract A novel concept was developed applying radioisotope-labelled substrate incorporation into the biomass. The resulting radiolabelled RNA was used both as an indicator of activity and as a template for gaining structural and functional information about a substrate-utilizing microbial community. Sequences of PCR products are separated via cloning or using molecular fingerprinting techniques. Nucleic acids from predominant clones or the whole molecular fingerprinting pattern are transferred to a membrane and hybridized with the radiolabelled sample RNA. Scanning of the hybridized blots for radioactivity indicates the members involved in the utilization of the substrate. This novel 'random walk' approach using radioisotope probing was evaluated in a model community experiment.
ID 2120
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2120
Nikolausz, M., Palatinszky, M., Rusznyák, A., Richnow, H.H., Kappelmeyer, U., Kästner, M. (2007):
Novel approach using substrate-mediated radiolabelling of RNA to link metabolic function with the structure of microbial communities
FEMS Microbiol. Lett. 274 (1), 154 - 161