Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Specific induction of the unique GPR15 expression in heterogeneous blood lymphocytes by tobacco smoking
Autor Bauer, M.; Hackermüller, J.; Schor, J.; Schreiber, S.; Fink, B.; Pierzchalski, A.; Herberth, G.;
Journal / Serie Biomarkers
Erscheinungsjahr 2019
Department IMMU; MOLSYB;
Band/Volume 24
Heft 3
Sprache englisch;
POF III (gesamt) F11; T42;
Keywords GPR15; MAIT; Th17; Treg; smoking
Abstract

Purpose: In the peripheral blood, it has been shown that smoking is, to date, the only specific condition leading to an increase in GPR15+ T cells. We, therefore, aimed to characterize GPR15-expressing blood T cells in more detail.

Materials and Methods: The whole transcriptome by RNAseq as a proxy for protein expression was analyzed in GPR15+ and GPR15- T cells. A deep immuno-phenotyping was conducted for the identification of T cell subtypes. Results: The expression of GPR15 seemed to be unique, not concomitantly accompanied with the expression of another protein. According to different T cell subtypes, there is no single cell type prominently represented in GPR15+ T cells. The individually different proportions of GPR15+ cells among each GPR15-expressing T cell subtypes in blood were strongly associated with chronic smoking. Indeed, the frequency of GPR15+ T cell subtypes can be effectively used as a highly convincing biomarker for tobacco smoking. Conclusions: While the chronic smoking-induced enrichment of GPR15+ T cells in blood might indicate a systemic inflammation, by the widespread presence in different T cell subtypes, GPR15 could feature a general impact on maintaining the systemic homeostasis to putatively prevent harm from smoking.

ID 21108
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21108
Bauer, M., Hackermüller, J., Schor, J., Schreiber, S., Fink, B., Pierzchalski, A., Herberth, G. (2019):
Specific induction of the unique GPR15 expression in heterogeneous blood lymphocytes by tobacco smoking
Biomarkers 24 (3), 217 - 224