Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Roquin targets mRNAs in a 3′-UTR-specific manner by different modes of regulation
Autor Essig, K.; Kronbeck, N.; Guimaraes, J.C.; Lohs, C.; Schlundt, A.; Hoffmann, A.; Behrens, G.; Brenner, S.; Kowalska, J.; Lopez-Rodriguez, C.; Jemielity, J.; Holtmann, H.; Reiche, K.; Hackermüller, J.; Sattler, M.; Zavolan, M.; Heissmeyer, V.;
Journal / Serie Nature Communications
Erscheinungsjahr 2018
Department MOLSYB;
Band/Volume 9
Sprache englisch;
POF III (gesamt) T42; F11;
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-018-06184-3/MediaObjects/41467_2018_6184_MOESM1_ESM.pdf
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-018-06184-3/MediaObjects/41467_2018_6184_MOESM3_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-018-06184-3/MediaObjects/41467_2018_6184_MOESM4_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-018-06184-3/MediaObjects/41467_2018_6184_MOESM5_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-018-06184-3/MediaObjects/41467_2018_6184_MOESM6_ESM.xlsx
Abstract The RNA-binding proteins Roquin-1 and Roquin-2 redundantly control gene expression and cell-fate decisions. Here, we show that Roquin not only interacts with stem–loop structures, but also with a linear sequence element present in about half of its targets. Comprehensive analysis of a minimal response element of the Nfkbid 3′-UTR shows that six stem–loop structures cooperate to exert robust and profound post-transcriptional regulation. Only binding of multiple Roquin proteins to several stem–loops exerts full repression, which redundantly involved deadenylation and decapping, but also translational inhibition. Globally, most Roquin targets are regulated by mRNA decay, whereas a small subset, including the Nfat5 mRNA, with more binding sites in their 3′-UTRs, are also subject to translational inhibition. These findings provide insights into how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements.
ID 20951
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20951
Essig, K., Kronbeck, N., Guimaraes, J.C., Lohs, C., Schlundt, A., Hoffmann, A., Behrens, G., Brenner, S., Kowalska, J., Lopez-Rodriguez, C., Jemielity, J., Holtmann, H., Reiche, K., Hackermüller, J., Sattler, M., Zavolan, M., Heissmeyer, V. (2018):
Roquin targets mRNAs in a 3′-UTR-specific manner by different modes of regulation
Nat. Commun. 9 , art. 3810