Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1074/jbc.M607159200
Titel (primär) The "jaws" of the tau-microtubule interaction
Autor Mukrasch, M.D.; von Bergen, M.; Biernat, J.; Fischer, D.; Griesinger, C.; Mandelkow, E.; Zweckstetter, M.
Quelle Journal of Biological Chemistry
Erscheinungsjahr 2007
Department PROTEOM
Band/Volume 282
Heft 16
Seite von 12230
Seite bis 12239
Sprache englisch
Abstract Tau is the major microtubule-associated protein in neuronal axons. It aggregates into "neurofibrillary tangles" during the course of Alzheimer disease. Binding to microtubules and microtubule assembly requires the "repeat domain" in the C-terminal half of Tau, as well as the two regions flanking the repeats. Here we report the NMR characterization of a 198-residue Tau fragment composed of the four tandem repeats and the flanking domains and containing the full microtubule binding and assembly activity of Tau. NMR secondary chemical shifts and dipolar couplings detect the highest propensity for -structure within the four-repeat region, whereas the flanking domains are largely random coil, with an increased rigidity in the proline-rich region. Chemical shift perturbation experiments identify two motifs in the upstream flanking domain, 225KVAVVRT231 and 243LQTA246, and one downstream of the repeats, 370KIETHKTFREN380, which strongly contribute to the binding to the acidic outside of microtubules, as well as to the binding of other polyanions such as heparin. This is consistent with the "jaws" model of Tau-microtubule interactions and highlights the importance of the regions flanking the repeats for both microtubule binding and pathological Tau aggregation.
dauerhafte UFZ-Verlinkung
Mukrasch, M.D., von Bergen, M., Biernat, J., Fischer, D., Griesinger, C., Mandelkow, E., Zweckstetter, M. (2007):
The "jaws" of the tau-microtubule interaction
J. Biol. Chem. 282 (16), 12230 - 12239 10.1074/jbc.M607159200