Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1371/journal.pone.0203087 |
Lizenz  |
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| Titel (primär) | An automated screening method for detecting compounds with goitrogenic activity using transgenic zebrafish embryos |
| Autor | Jarque, S.; Fetter, E.; Veneman, W.J.; Spaink, H.P.; Peravali, R.; Strähle, U.; Scholz, S.
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| Quelle | PLOS ONE |
| Erscheinungsjahr | 2018 |
| Department | BIOTOX |
| Band/Volume | 13 |
| Heft | 8 |
| Seite von | e0203087 |
| Sprache | englisch |
| Supplements | Supplement 1 |
| Keywords | Embryos; Thyroid; Zebrafish; Fluorescence imaging; Perchlorates; Toxicity; Image analysis; Thyroid hormones |
| Abstract | The knowledge on environmentally relevant chemicals that may interfere with thyroid signaling is scarce. Here, we present a method for the screening of goitrogens, compounds that disrupt the thyroid gland function, based on the automatic orientation of zebrafish in a glass capillary and a subsequent imaging of reporter gene fluorescence in the thyroid gland of embryos of the transgenic zebrafish line tg(tg:mCherry). The tg(tg:mCherry) reporter gene indicates a compensatory upregulation of thyroglobulin, the thyroid hormone precursor, in response to inhibition of thyroid hormone synthesis. Fish embryos were exposed to a negative control compound (3,4-dichloroaniline), or a concentration series of known goitrogenic compounds (resorcinol, methimazole, potassium perchlorate, 6-propyl-2-thiouracil, ethylenethiourea, phloroglucinol, pyrazole) with maximum exposure concentration selected based on mortality and/or solubility. Exposure to 3,4-dichloroaniline decreased the fluorescence signal. All goitrogenic compounds exhibited clear concentration-dependent inductions of reporter fluorescence 1.4 to 2.6 fold above control levels. Concentration-response modelling was used to calculate goitrogenic potencies based on EC50 values. The new automated method offers an efficient screening approach for goitrogenic activity. |
| Jarque, S., Fetter, E., Veneman, W.J., Spaink, H.P., Peravali, R., Strähle, U., Scholz, S. (2018): An automated screening method for detecting compounds with goitrogenic activity using transgenic zebrafish embryos PLOS One 13 (8), e0203087 10.1371/journal.pone.0203087 |
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