Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Creative Commons Lizenz creative commons licence
Titel (primär) An automated screening method for detecting compounds with goitrogenic activity using transgenic zebrafish embryos
Autor Jarque, S.; Fetter, E.; Veneman, W.J.; Spaink, H.P.; Peravali, R.; Strähle, U.; Scholz, S.;
Journal / Serie PLOS ONE
Erscheinungsjahr 2018
Department BIOTOX;
Band/Volume 13
Heft 8
Sprache englisch;
POF III (gesamt) T42;
Supplements https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203087#sec013
Keywords Embryos; Thyroid; Zebrafish; Fluorescence imaging; Perchlorates; Toxicity; Image analysis; Thyroid hormones
Abstract The knowledge on environmentally relevant chemicals that may interfere with thyroid signaling is scarce. Here, we present a method for the screening of goitrogens, compounds that disrupt the thyroid gland function, based on the automatic orientation of zebrafish in a glass capillary and a subsequent imaging of reporter gene fluorescence in the thyroid gland of embryos of the transgenic zebrafish line tg(tg:mCherry). The tg(tg:mCherry) reporter gene indicates a compensatory upregulation of thyroglobulin, the thyroid hormone precursor, in response to inhibition of thyroid hormone synthesis. Fish embryos were exposed to a negative control compound (3,4-dichloroaniline), or a concentration series of known goitrogenic compounds (resorcinol, methimazole, potassium perchlorate, 6-propyl-2-thiouracil, ethylenethiourea, phloroglucinol, pyrazole) with maximum exposure concentration selected based on mortality and/or solubility. Exposure to 3,4-dichloroaniline decreased the fluorescence signal. All goitrogenic compounds exhibited clear concentration-dependent inductions of reporter fluorescence 1.4 to 2.6 fold above control levels. Concentration-response modelling was used to calculate goitrogenic potencies based on EC50 values. The new automated method offers an efficient screening approach for goitrogenic activity.
ID 20854
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20854
Jarque, S., Fetter, E., Veneman, W.J., Spaink, H.P., Peravali, R., Strähle, U., Scholz, S. (2018):
An automated screening method for detecting compounds with goitrogenic activity using transgenic zebrafish embryos
PLOS One 13 (8), e0203087