Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) An improved strategy for selective and efficient enrichment of integral plasma membrane proteins of mycobacteria
Autor Mattow, J.; Siejak, F.; Hagens, K.; Schmidt, F.; Koehler, C.; Treumann, A.; Schaible, U.E.; Kaufmann, S.H.E.;
Journal / Serie Proteomics
Erscheinungsjahr 2007
Department PROTEOM;
Band/Volume 7
Heft 10
Sprache englisch;
Keywords integral plasma membrane proteins; Mycobacteria; Mycobacterium bovis; Mycobacterium tuberculosis; prefractionation method
Abstract Mycobacterial plasma membrane proteins play essential roles in many cellular processes, yet their comprehensive proteomic profiling remains challenging. This is mainly due to obstacles related to their extraction and solubilization. To tackle this problem, we have developed a novel procedure to selectively enrich mycobacterial. plasma membrane proteins based on alkaline sodium carbonate washing of crude membranes followed by Triton X-114 phase partitioning. The present study assesses the efficiency of this method by proteome analysis of plasma membrane proteins from Mycobacterium bovis BCG. Extracted proteins were separated in parallel by 1-D SDS-PAGE and 2-DE and then analyzed by LC-MS/MS and MALDI-MS/MS. Our study revealed 125 proteins, of which 54 contained 1-14 predicted transmembrane domains (TMD) including nine novel proteins. The 1-D SDS-PAGE-based proteome analysis identified 81 proteins, of which 49 (60.5%) harbored TMD. This approach also revealed many hydrophobic membrane-associated/periplasmic proteins lacking TMD, but only few soluble proteins. The identified proteins were characterized with regard to biological functions and physicochemical properties providing further evidence for the high efficiency of the prefractionation. method described herein
ID 2051
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2051
Mattow, J., Siejak, F., Hagens, K., Schmidt, F., Koehler, C., Treumann, A., Schaible, U.E., Kaufmann, S.H.E. (2007):
An improved strategy for selective and efficient enrichment of integral plasma membrane proteins of mycobacteria
Proteomics 7 (10), 1687 - 1701