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Titel (primär) High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations
Autor Russel, M.; Sophocleous, M.; JiaJia, S.; Xu, W.; Xiao, L.; Maskow, T.; Alam, M.; Georgiou, J.;
Journal / Serie Analytica Chimica Acta
Erscheinungsjahr 2018
Department UMB;
Band/Volume 1028
Sprache englisch;
POF III (gesamt) T41;
Keywords Dielectric spectroscopy; Complex permittivity; Bacterial monitoring; Biophycial; Pathogen monitoring
Abstract This paper describes a novel technique to quantify and identify bacterial cultures of Bacillus Subtilis (2.10–1.30 × 109 CFU mL−1) and Escherichia Coli (1.60–1.00 × 109 CFU mL−1), in corn oil using dielectric spectroscopy at elevated frequencies of 0.0100–20.0 GHz. This technique is using the electrophysiological/biophysical differences (e.g. gram positive and gram negative) between various bacteria types, as a basis to distinguish between bacteria concentrations and bacteria types. A close-ended, coaxial probe of 20.0 mm long sample-holder was developed and used to calculate the dielectric constant from the measured S parameters of the bacterial cultures, using the Nicolson-Ross-Weir method. This technique shows a linear relationship (r2 ≥ 0.999) between the dielectric constant and the cell concentration, at 16.0 GHz. The sensitivity of the technique is 0.177 × 109 (CFU mL−1)−1 for B. Subtilis (with a size of 10.0 × 1.00 μm), 0.322 × 109 (CFU mL−1)−1 for E. Coli (with a size of 2.00 × 0.500 μm) and 0.913 × 109 (CFU mL−1) −1 for their 1:1 mixture, while the response time is 60.0s. The dependency of dielectric constant on the bacterial cell concentration at a given frequency can be potentially exploited for measuring bacterial concentrations and biophysical differences.
ID 20477
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=20477
Russel, M., Sophocleous, M., JiaJia, S., Xu, W., Xiao, L., Maskow, T., Alam, M., Georgiou, J. (2018):
High-frequency, dielectric spectroscopy for the detection of electrophysiological/biophysical differences in different bacteria types and concentrations
Anal. Chim. Acta 1028 , 86 - 95