Details zur Publikation
|DOI / URL||Link|
|Titel (primär)||Proteome and phosphoproteome analysis of commensally induced dendritic cell maturation states|
|Autor||Korkmaz, A.G.; Popov, I.; Peisl, L.; Codrea, M.C.; Nahnsen, S.; Steimle, A.; Velic, A.; Macek, B.; von Bergen, M.; Bernhardt, J.; Frick, J.S.;|
|Journal / Serie||Journal of Proteomics|
|POF III (gesamt)||F11;|
|Keywords||Commensals; B. vulgatus; Dendritic cells; Inflammation; Shotgun proteomics; Expression analysis|
Dendritic cells (DCs) can shape the immune system towards an inflammatory or tolerant state depending on the bacterial antigens and the environment they encounter. In this study we provide a proteomic catalogue of differentially expressed proteins between distinct DC maturation states, brought about by bacteria that differ in their endotoxicity. To achieve this, we have performed proteomics and phosphoproteomics on murine DC cultures. Symbiont and pathobiont bacteria were used to direct dendritic cells into a semi-mature and fully-mature state, respectively. The comparison of semi-mature and fully-mature DCs revealed differential expression in 103 proteins and differential phosphorylation in 118 phosphosites, including major regulatory factors of central immune processes. Our analyses predict that these differences are mediated by upstream elements such as SOCS1, IRF3, ABCA1, TLR4, and PTGER4. Our analyses indicate that the symbiont bacterial strain affects DC proteome in a distinct way, by downregulating inflammatory proteins and activating anti-inflammatory upstream regulators.
In this study we have investigated the responses of immune cells to distinct bacterial stimuli. We have used the symbiont bacterial strain B. vulgatus and the pathobiont E. coli strain to stimulate cultured primary dendritic cells and performed a shotgun proteome analysis to investigate the protein expression and phosphorylation level differences on a genome level. We have observed expression and phosphorylation level differences in key immune regulators, transcription factors and signal transducers. Moreover, our subsequent bioinformatics analysis indicated regulation at several signaling pathways such as PPAR signaling, LXR/RXR activation and glucocorticoid signaling pathways, which are not studied in detail in an inflammation and DC maturation context. Our phosphoproteome analysis showed differential phosphorylation in 118 phosphosites including those belonging to epigenetic regulators, transcription factors and major cell cycle regulators. We anticipate that our study will facilitate further investigation of immune cell proteomes under different inflammatory and non-inflammatory conditions.
|Korkmaz, A.G., Popov, I., Peisl, L., Codrea, M.C., Nahnsen, S., Steimle, A., Velic, A., Macek, B., von Bergen, M., Bernhardt, J., Frick, J.S. (2018):
Proteome and phosphoproteome analysis of commensally induced dendritic cell maturation states
J. Proteomics 180 , 11 - 24