Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1002/pmic.200600848 |
| Titel (primär) | Comparison of lung proteome profiles in two rodent models of pulmonary arterial hypertension |
| Autor | Laudi, S.; Steudel, W.; Jonscher, K.; Schöning, W.; Schniedewind, B.; Kaisers, U.; Christians, U.; Trump, S. |
| Quelle | Proteomics |
| Erscheinungsjahr | 2007 |
| Department | IMMU |
| Band/Volume | 7 |
| Heft | 14 |
| Seite von | 2469 |
| Seite bis | 2478 |
| Sprache | englisch |
| Keywords | Hypoxia; Monocrotaline; Protein expression analysis; Pulmonary arterial hypertension |
| Abstract | We studied the lung proteome changes in two widely used models of pulmonary arterial hypertension (PAH): monocrotaline (MCT) injection and chronic hypoxia (CH); untreated rats were used as controls (n = 6/group). After 28 days, invasive right ventricular systolic pressure (RVSP) was measured. Lungs were immunostained for -smooth muscle actin (SMA). 2-DE (n = 4/group) followed by nano-LC-MS/MS was applied for protein identification. Western blotting was used additionally if possible. RVSP was significantly increased in MCT- and CH-rats (MCT 62.5 ± 4.4 mmHg, CH 62.2 ± 4.1 mmHg, control 25.0 ± 1.7 mmHg, p<0.001). This was associated with an increase of SMA positive vessels. In both groups, there was a significantly increased expression of proteins associated with the contractile apparatus (diphosphoHsp27 (p<0.001), Septin2 (p<0.001), F-actin capping protein (p<0.01), and tropomyosin (p<0.02)). In CH, proteins of the nitric oxide (Hsc70; p = 0.002), carbon monoxide (biliverdin reductase; p = 0.005), and vascular endothelial growth factor (VEGF) pathway (annexin 3; p<0.001) were significantly increased. In MCT, proteins involved in serotonin synthesis (14-3-3; p = 0.02), the enhanced unfolded protein response (ERp57; p = 0.02), and intracellular chloride channels (CLIC 1; p = 0.002) were significantly elevated. Therefore, MCT- and CH-induced vasoconstriction and remodeling seemed to be mediated via different signaling pathways. These differences should be considered in future studies using either PAH model. |
| Laudi, S., Steudel, W., Jonscher, K., Schöning, W., Schniedewind, B., Kaisers, U., Christians, U., Trump, S. (2007): Comparison of lung proteome profiles in two rodent models of pulmonary arterial hypertension Proteomics 7 (14), 2469 - 2478 10.1002/pmic.200600848 |
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