Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1038/s41467-017-02582-1 |
Lizenz  |
|
| Titel (primär) | Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA |
| Autor | Rehage, N.; Davydova, E.; Conrad, C.; Behrens, G.; Maiser, A.; Stehklein, J.E.; Brenner, S.; Klein, J.; Jeridi, A.; Hoffmann, A.; Lee, E.; Dianzani, U.; Willemsen, R.; Feederle, R.; Reiche, K.; Hackermüller, J.
; Leonhardt, H.; Sharma, S.; Niessing, D.; Heissmeyer, V. |
| Quelle | Nature Communications |
| Erscheinungsjahr | 2018 |
| Department | MOLSYB |
| Band/Volume | 9 |
| Seite von | art. 299 |
| Sprache | englisch |
| Supplements | https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-017-02582-1/MediaObjects/41467_2017_2582_MOESM1_ESM.pdf https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-017-02582-1/MediaObjects/41467_2017_2582_MOESM2_ESM.pdf https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-017-02582-1/MediaObjects/41467_2017_2582_MOESM3_ESM.pdf https://static-content.springer.com/esm/art%3A10.1038%2Fs41467-017-02582-1/MediaObjects/41467_2017_2582_MOESM4_ESM.xlsx |
| UFZ Querschnittsthemen | RU3; |
| Abstract | The ubiquitously expressed RNA-binding proteins Roquin-1 and Roquin-2 are essential for appropriate immune cell function and postnatal survival of mice. Roquin proteins repress target mRNAs by recognizing secondary structures in their 3′-UTRs and by inducing mRNA decay. However, it is unknown if other cellular proteins contribute to target control. To identify cofactors of Roquin, we used RNA interference to screen ~1500 genes involved in RNA-binding or mRNA degradation, and identified NUFIP2 as a cofactor of Roquin-induced mRNA decay. NUFIP2 binds directly and with high affinity to Roquin, which stabilizes NUFIP2 in cells. Post-transcriptional repression of human ICOS by endogenous Roquin proteins requires two neighboring non-canonical stem-loops in the ICOS 3′-UTR. This unconventional cis-element as well as another tandem loop known to confer Roquin-mediated regulation of the Ox40 3′-UTR, are bound cooperatively by Roquin and NUFIP2. NUFIP2 therefore emerges as a cofactor that contributes to mRNA target recognition by Roquin. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19823 |
| Rehage, N., Davydova, E., Conrad, C., Behrens, G., Maiser, A., Stehklein, J.E., Brenner, S., Klein, J., Jeridi, A., Hoffmann, A., Lee, E., Dianzani, U., Willemsen, R., Feederle, R., Reiche, K., Hackermüller, J., Leonhardt, H., Sharma, S., Niessing, D., Heissmeyer, V. (2018): Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA Nat. Commun. 9 , art. 299 10.1038/s41467-017-02582-1 |
|