Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.scitotenv.2017.12.127 |
| Volltext | Autorenversion |
| Titel (primär) | Characterisation of electron beam irradiation-immobilised laccase for application in wastewater treatment |
| Autor | Jahangiri, E.; Thomas, I.; Schulze, A.; Seiwert, B.; Cabana, H.; Schlosser, D.
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| Quelle | Science of the Total Environment |
| Erscheinungsjahr | 2018 |
| Department | UMB; ANA |
| Band/Volume | 624 |
| Seite von | 309 |
| Seite bis | 322 |
| Sprache | englisch |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S0048969717335532-mmc1.pdf |
| Keywords | Acetaminophen; Laccase immobilisation; Micro-pollutants; Pharmaceutically active compounds; Transformation products; Wastewater |
| UFZ Querschnittsthemen | RU3; |
| Abstract | Laccase from Phoma sp. UHH 5-1-03 was cross-linked to polyvinylidene fluoride membranes by electron beam irradiation. Immobilised laccase displayed a higher stability than the non-immobilised enzyme with respect to typical wastewater temperatures, and pH at a range of 5 to 9. Batch tests addressed the removal of pharmaceutically active compounds (PhACs; applied as a mixture of acetaminophen, bezafibrate, indometacin, ketoprofen, mefenamic acid, and naproxen) by both immobilised and non-immobilised laccase in municipal wastewater. High removal rates (> 85%) of the most efficiently oxidised PhACs (acetaminophen and mefenamic acid) indicated a high efficiency of the immobilised laccase in wastewater. Continuous elimination of the aforementioned PhACs by the immobilised enzyme in a continuously operated diffusion basket reactor yielded a PhAC removal pattern qualitatively similar to those observed in batch tests. Clearly higher apparent Vmax values and catalytic efficiencies (in terms of both Vmax/S0.5 as well as Vmax/Km values obtained from data fitting according to the Hill and the Michaelis-Menten model, respectively) observed for acetaminophen oxidation by the immobilised compared to the non-immobilised enzyme are in support of a considerably higher functional stability of the immobilised laccase especially in wastewater. The potential influence of acetaminophen on the removal of comparatively less laccase-oxidisable water pollutants such as the antimicrobial triclosan (TCS) was investigated. TCS was increasingly removed upon increasing the initial acetaminophen concentration in immobilised as well as non-immobilised laccase reaction systems until saturation became evident. Acetaminophen was consumed and not recycled during laccase reactions, which was accompanied by the formation of various acetaminophen-TCS cross-coupling products. Nevertheless, the simultaneous presence of acetaminophen (and potentially even more pollutant removal-enhancing laccase substrates) and more recalcitrant pollutants in wastewater represents an interesting option for the efficiency enhancement of enzyme-based wastewater treatment approaches. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19744 |
| Jahangiri, E., Thomas, I., Schulze, A., Seiwert, B., Cabana, H., Schlosser, D. (2018): Characterisation of electron beam irradiation-immobilised laccase for application in wastewater treatment Sci. Total Environ. 624 , 309 - 322 10.1016/j.scitotenv.2017.12.127 |
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