Details zur Publikation
|DOI / URL||Link|
|Titel (primär)||Balancing cellular redox metabolism in microbial electrosynthesis and electro fermentation – A chance for metabolic engineering|
|Autor||Kracke, F.; Lai, B.; Yu, S.; Krömer, J.O.|
|Journal / Serie||Metabolic Engineering|
|Keywords||Microbial electrosynthesis; Electro-fermentation; Extracellular electron transfer; Redox reactions; Electron transport pathway engineering|
More and more microbes are discovered that are capable of extracellular electron transfer, a process in which they use external electrodes as electron donors or acceptors for metabolic reactions. This feature can be used to overcome cellular redox limitations and thus optimizing microbial production. The technologies, termed microbial electrosynthesis and electro-fermentation, have the potential to open novel bio-electro production platforms from sustainable energy and carbon sources. However, the performance of reported systems is currently limited by low electron transport rates between microbes and electrodes and our limited ability for targeted engineering of these systems due to remaining knowledge gaps about the underlying fundamental processes.
Metabolic engineering offers many opportunities to optimize these processes, for instance by genetic engineering of pathways for electron transfer on the one hand and target product synthesis on the other hand. With this review, we summarize the status quo of knowledge and engineering attempts around chemical production in bio-electrochemical systems from a microbe perspective. Challenges associated with the introduction or enhancement of extracellular electron transfer capabilities into production hosts versus the engineering of target compound synthesis pathways in natural exoelectrogens are discussed. Recent advances of the research community in both directions are examined critically. Further, systems biology approaches, for instance using metabolic modelling, are examined for their potential to provide insight into fundamental processes and to identify targets for metabolic engineering.
|Kracke, F., Lai, B., Yu, S., Krömer, J.O. (2018):
Balancing cellular redox metabolism in microbial electrosynthesis and electro fermentation – A chance for metabolic engineering
Metab. Eng. 45 , 109 - 120