Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00449-017-1785-z
Volltext Shareable Link
Titel (primär) Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae
Autor Averesch, N.J.H.; Prima, A.; Krömer, J.O.
Quelle Bioprocess and Biosystems Engineering
Erscheinungsjahr 2017
Department SOMA
Band/Volume 40
Heft 8
Seite von 1283
Seite bis 1289
Sprache englisch
Keywords Para-hydroxybenzoic acid; Shikimate pathway; Saccharomyces cerevisiae; Fed-batch
UFZ Querschnittsthemen RU4;
Abstract Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized. Addressing both we show that S. cerevisiae is well-suited for over-production of aromatic compounds, which are valuable in chemical industry and are particularly useful in space technology. Specifically, a strain engineered to accumulate chorismate was optimized for formation of para-hydroxybenzoic acid. Then a fed-batch bioreactor process was developed, which delivered a final titer of 2.9 g/L, a maximum rate of 18.625 mgpHBA/(gCDW × h) and carbon-yields of up to 3.1 mgpHBA/gglucose.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=19009
Averesch, N.J.H., Prima, A., Krömer, J.O. (2017):
Enhanced production of para-hydroxybenzoic acid by genetically engineered Saccharomyces cerevisiae
Bioprocess. Biosyst. Eng. 40 (8), 1283 - 1289 10.1007/s00449-017-1785-z