Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1676/16-062.1 |
Titel (primär) | Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae) |
Autor | Menger, J.; Gerth, M.; Unrein, J.; Henle, K.; Schlegel, M. |
Quelle | Wilson Journal of Ornithology |
Erscheinungsjahr | 2017 |
Department | NSF; iDiv |
Band/Volume | 129 |
Heft | 2 |
Seite von | 407 |
Seite bis | 411 |
Sprache | englisch |
Keywords | Amazonia, ant-following birds, cross-amplification, next-generation sequencing, nuclear markers |
UFZ Querschnittsthemen | RU1 |
Abstract | Fourteen microsatellite loci were characterized for the Rufous-throated Antbird (Thamnophilidae: Gymnopithys rufigula), an Amazonian obligate ant-following bird. Eight novel polymorphic microsatellite loci were isolated using next-generation sequencing and six additional loci were cross-amplified using primers previously designed for other bird species. All 14 loci were screened using 40 samples from central Amazon. The number of alleles per locus ranged from 3 to 20, whilst the observed and expected heterozygosities varied from 0.200 to 0.975, and 0.186 to 0.928, respectively. No linkage disequilibrium was found among the loci. Only one locus deviated from Hardy-Weinberg equilibrium. Among the eight novel loci, six were cross-amplified in at least one additional Thamnophilidae species, whilst three loci cross-amplified in two other Dendrocolaptidae species. This set of markers will be useful for future population genetic structure and parentage analysis of Amazonian forest birds. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18994 |
Menger, J., Gerth, M., Unrein, J., Henle, K., Schlegel, M. (2017): Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae) Wilson J. Ornithol. 129 (2), 407 - 411 10.1676/16-062.1 |