Details zur Publikation

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Titel (primär) Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
Autor Menger, J.; Gerth, M.; Unrein, J.; Henle, K.; Schlegel, M.;
Journal / Serie Wilson Journal of Ornithology
Erscheinungsjahr 2017
Department NSF; iDiv;
Band/Volume 129
Heft 2
Sprache englisch;
POF III (gesamt) T12;
Keywords Amazonia, ant-following birds, cross-amplification, next-generation sequencing, nuclear markers
UFZ Querschnittsthemen RU1
Abstract Fourteen microsatellite loci were characterized for the Rufous-throated Antbird (Thamnophilidae: Gymnopithys rufigula), an Amazonian obligate ant-following bird. Eight novel polymorphic microsatellite loci were isolated using next-generation sequencing and six additional loci were cross-amplified using primers previously designed for other bird species. All 14 loci were screened using 40 samples from central Amazon. The number of alleles per locus ranged from 3 to 20, whilst the observed and expected heterozygosities varied from 0.200 to 0.975, and 0.186 to 0.928, respectively. No linkage disequilibrium was found among the loci. Only one locus deviated from Hardy-Weinberg equilibrium. Among the eight novel loci, six were cross-amplified in at least one additional Thamnophilidae species, whilst three loci cross-amplified in two other Dendrocolaptidae species. This set of markers will be useful for future population genetic structure and parentage analysis of Amazonian forest birds.
ID 18994
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18994
Menger, J., Gerth, M., Unrein, J., Henle, K., Schlegel, M. (2017):
Isolation and characterization of pmlymorphic Microsatellite Loci from the Rufous-throated Antbird Gymnopithys rufigula (Aves: Thamnophilidae)
Wilson J. Ornithol. 129 (2), 407 - 411