Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains
Autor Mortan, S.H.; Martín-González, L.; Vicent, T.; Caminal, G.; Nijenhuis, I.; Adrian, L.; Marco-Urrea, E.;
Journal / Serie Journal of Hazardous Materials
Erscheinungsjahr 2017
Department ISOBIO;
Band/Volume 331
Sprache englisch;
POF III (gesamt) T41;
Keywords Organohalide-respiring bacteria; Fed-batch reactor; Dehalococcoides; Dehalogenimonas; 1,1,2-Trichloroethane
UFZ Querschnittsthemen RU3;
Abstract 1,1,2-Trichloroethane (1,1,2-TCA) is a non-flammable organic solvent and common environmental contaminant in groundwater. Organohalide-respiring bacteria are key microorganisms to remediate 1,1,2-TCA because they can gain metabolic energy during its dechlorination under anaerobic conditions. However, all current isolates produce hazardous end products such as vinyl chloride, monochloroethane or 1,2-dichloroethane that accumulate in the medium. Here, we constructed a syntrophic co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains to achieve complete detoxification of 1,1,2-TCA to ethene. In this co-culture, Dehalogenimonas transformed 1,1,2-TCA via dihaloelimination to vinyl chloride, whereas Dehalococcoides reduced vinyl chloride via hydrogenolysis to ethene. Molasses, pyruvate, and lactate supported full dechlorination of 1,1,2-TCA in serum bottle co-cultures. Scale up of the cultivation to a 5-L bioreactor operating for 76 d in fed-batch mode was successful with pyruvate as substrate. This synthetic combination of bacteria with known complementary metabolic capabilities demonstrates the potential environmental relevance of microbial cooperation to detoxify 1,1,2-TCA.
ID 18557
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18557
Mortan, S.H., Martín-González, L., Vicent, T., Caminal, G., Nijenhuis, I., Adrian, L., Marco-Urrea, E. (2017):
Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains
J. Hazard. Mater. 331 , 218 - 225