Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00253-017-8128-5
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Titel (primär) Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype
Autor Perruchon, C.; Chatzinotas, A.; Omirou, M.; Vasileiadis, S.; Menkissoglou-Spiroudi, U.; Karpouzas, D.G.
Quelle Applied Microbiology and Biotechnology
Erscheinungsjahr 2017
Department UMB; iDiv
Band/Volume 101
Heft 9
Seite von 3881
Seite bis 3893
Sprache englisch
Keywords Thiabendazole; Fruit-packaging industry; Wastewaters; SIP-DGGE; Pesticide biodegradation; Sphingomonas
UFZ Querschnittsthemen RU1;
Abstract Thiabendazole (TBZ) is a fungicide used in fruit-packaging plants. Its application leads to the production of wastewaters requiring detoxification. In the absence of efficient treatment methods, biological depuration of these effluents could be a viable alternative. However, nothing is known regarding the microbial degradation of the recalcitrant and toxic to aquatics TBZ. We report the isolation, via enrichment cultures from a polluted soil, of the first bacterial consortium able to rapidly degrade TBZ and use it as a carbon source. Repeated efforts using various culture-dependent approaches failed to isolate TBZ-degrading bacteria in axenic cultures. Denaturating gradient gel electrophoresis (DGGE) and cloning showed that the consortium was composed of α-, β- and γ-Proteobacteria. Culture-independent methods including antibiotics-driven selection with DNA/RNA-DGGE, q-PCR and stable isotope probing (SIP)-DGGE identified a Sphingomonas phylotype (B13) as the key degrading member. Cross-feeding studies with structurally related chemicals showed that ring substituents of the benzimidazole moiety (thiazole or furan rings) favoured the cleavage of the imidazole moiety. LC-MS/MS analysis verified that TBZ degradation proceeds via cleavage of the imidazole moiety releasing thiazole-4-carboxamidine, which was not further transformed, and the benzoyl moiety, possibly as catechol, which was eventually consumed by the bacterial consortium as suggested by SIP-DGGE.
dauerhafte UFZ-Verlinkung
Perruchon, C., Chatzinotas, A., Omirou, M., Vasileiadis, S., Menkissoglou-Spiroudi, U., Karpouzas, D.G. (2017):
Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype
Appl. Microbiol. Biotechnol. 101 (9), 3881 - 3893 10.1007/s00253-017-8128-5