Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Multistage bioassociation of uranium onto an extremely halophilic archaeon revealed by a unique combination of spectroscopic and microscopic techniques
Autor Bader, M.; Müller, K.; Foerstendorf, H.; Drobot, B.; Schmidt, M.; Musat, N.; Swanson, J.S.; Reed, D.T.; Stumpf, T.; Cherkouk, A.;
Journal / Serie Journal of Hazardous Materials
Erscheinungsjahr 2017
Department ISOBIO;
Band/Volume 327
Sprache englisch;
POF III (gesamt) T15; T41;
Keywords Uranium bioassociation; Halophilic archaeon Halobacterium noricense; In situ ATR FT-IR spectroscopy; Luminescence spectroscopy at high salinity; Final disposal of radioactive waste
UFZ Querschnittsthemen RU4;
Abstract The interactions of two extremely halophilic archaea with uranium were investigated at high ionic strength as a function of time, pH and uranium concentration. Halobacterium noricense DSM-15987 and Halobacterium sp. putatively noricense, isolated from the Waste Isolation Pilot Plant repository, were used for these investigations. The kinetics of U(VI) bioassociation with both strains showed an atypical multistage behavior, meaning that after an initial phase of U(VI) sorption, an unexpected interim period of U(VI) release was observed, followed by a slow reassociation of uranium with the cells. By applying in situ attenuated total reflection Fourier-transform infrared spectroscopy, the involvement of phosphoryl and carboxylate groups in U(VI) complexation during the first biosorption phase was shown. Differences in cell morphology and uranium localization become visible at different stages of the bioassociation process, as shown with scanning electron microscopy in combination with energy dispersive X-ray spectroscopy. Our results demonstrate for the first time that association of uranium with the extremely halophilic archaeon is a multistage process, beginning with sorption and followed by another process, probably biomineralization.
ID 18530
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=18530
Bader, M., Müller, K., Foerstendorf, H., Drobot, B., Schmidt, M., Musat, N., Swanson, J.S., Reed, D.T., Stumpf, T., Cherkouk, A. (2017):
Multistage bioassociation of uranium onto an extremely halophilic archaeon revealed by a unique combination of spectroscopic and microscopic techniques
J. Hazard. Mater. 327 , 225 - 232