Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.scitotenv.2016.11.154
Titel (primär) Effects of chronic dietary exposure of zinc oxide nanoparticles on the serum protein profile of juvenile common carp (Cyprinus carpio L.)
Autor Chupani, L.; Zusková, É.; Niksirat, H.; Panáček, A.; Lünsmann, V.; Haange, S.-B. ORCID logo ; von Bergen, M.; Jehmlich, N. ORCID logo
Quelle Science of the Total Environment
Erscheinungsjahr 2017
Department MOLSYB
Band/Volume 579
Seite von 1504
Seite bis 1511
Sprache englisch
Keywords Common carp; Dietary exposure; Nanoparticle; Proteomics; Serum; Zinc oxide
UFZ Querschnittsthemen RU3;
Abstract Zinc oxide (ZnO) nanoparticles (NPs) have been dramatically used in industry, biology, and medicine. Despite their interesting physico-chemical properties for application in various industrial, medical, and consumer products, safe use of ZnO NPs are under challenges due to the inadequate information related to their toxicological endpoints. Proteomics was applied to evaluate the sub-lethal effects of dietary exposure to ZnO NPs on serum proteome profile of juvenile common carp, (Cyprinus carpio). Therefore, ZnO NPs solution (500 mg kg− 1 of feed) was added to a commercial carp feed for six weeks. We compared the serum proteome profile from 7 controls and 7 treated fish. In addition, zinc accumulation were measured in intestine, liver, gill and brain. In total, we were able to identify 326 proteins from 6845 distinct peptides. As a result of the data analysis, the abundance levels of four proteins were significantly altered (fold change (fc) ≥ 2 and p < 0.05) after dietary exposure to ZnO NPs. The protein levels of the complement component C4-2 (fc 2.5) and the uncharacterised protein encoded by kng1 (fc 5.8) were increased and major histocompatibility class I (fc 4.9) and the uncharacterised protein encoded by lum (fc 3.5) were decreased (fc 2.5). Molecular pathway analysis revealed four canonical pathways including acute-phase response signalling, liver and retinoid X receptors activation, and intrinsic and extrinsic prothrombin activation pathways as significantly regulated in the treated fish. No significant difference was observed for zinc accumulation in exposed fish compared to controls. In summary, despite no apparent accumulation, ZnO NPs exposure to common carp probably disturbs the fish homeostasis by affecting proteins of the haematological and the immune systems.
dauerhafte UFZ-Verlinkung
Chupani, L., Zusková, É., Niksirat, H., Panáček, A., Lünsmann, V., Haange, S.-B., von Bergen, M., Jehmlich, N. (2017):
Effects of chronic dietary exposure of zinc oxide nanoparticles on the serum protein profile of juvenile common carp (Cyprinus carpio L.)
Sci. Total Environ. 579 , 1504 - 1511 10.1016/j.scitotenv.2016.11.154