Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s10295-016-1890-z
Volltext Shareable Link
Titel (primär) Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing
Autor Kadisch, M.; Schmid, A.; Bühler, B.
Journal / Serie Journal of Industrial Microbiology & Biotechnology
Erscheinungsjahr 2017
Department SOMA
Band/Volume 44
Heft 3
Seite von 339
Seite bis 351
Sprache englisch
Keywords BioH; Fatty acid methyl ester hydrolysis; Whole-cell biocatalysis; Industrial biotechnology; Metabolic engineering
UFZ Querschnittsthemen RU4;
Abstract Fatty acid methyl esters (FAMEs) originating from plant oils are most interesting renewable feedstocks for biofuels and bio-based materials. FAMEs can also be produced and/or functionalized by engineered microbes to give access to, e.g., polymer building blocks. Yet, they are often subject to hydrolysis yielding free fatty acids, which typically are degraded by microbes. We identified BioH as the key enzyme responsible for the hydrolysis of medium-chain length FAME derivatives in different E. coli K-12 strains. E. coli ΔbioH strains showed up to 22-fold reduced FAME hydrolysis rates in comparison with respective wild-type strains. Knockout strains showed, beside the expected biotin auxotrophy, unchanged growth behavior and biocatalytic activity. Thus, high specific rates (~80 U gCDW−1) for terminal FAME oxyfunctionalization catalyzed by a recombinant alkane monooxygenase could be combined with reduced hydrolysis. Biotransformations in process-relevant two-liquid phase systems profited from reduced fatty acid accumulation and/or reduced substrate loss via free fatty acid metabolization. The BioH knockout strategy was beneficial in all tested strains, although its effect was found to differ according to specific strain properties, such as FAME hydrolysis and FFA degradation activities. BioH or functional analogs can be found in virtually all microorganisms, making bioH deletion a broadly applicable strategy for efficient microbial bioprocessing involving FAMEs.
dauerhafte UFZ-Verlinkung
Kadisch, M., Schmid, A., Bühler, B. (2017):
Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing
J. Ind. Microbiol. Biotechnol. 44 (3), 339 - 351