Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.envpol.2016.11.011
Titel (primär) Impact of untreated wastewater on a major European river evaluated with a combination of in vitro bioassays and chemical analysis
Autor König, M.; Escher, B.I.; Neale, P.A.; Krauss, M. ORCID logo ; Hilscherová, K.; Novák, J.; Teodorović, I.; Schulze, T. ORCID logo ; Seidensticker, S.; Hashmi, M.A.K.; Ahlheim, J.; Brack, W.
Quelle Environmental Pollution
Erscheinungsjahr 2017
Department WANA; ZELLTOX
Band/Volume 220
Heft Part B
Seite von 1220
Seite bis 1230
Sprache englisch
Keywords Bioassay; Xenobiotic metabolism; Specific modes of action; Adaptive stress response; Bioanalytical equivalent concentration
UFZ Querschnittsthemen RU2;
Abstract Complex mixtures of micropollutants, including pesticides, pharmaceuticals and industrial chemicals emitted by wastewater effluents to European rivers may compromise the quality of these water resources and may pose a risk to ecosystem health and abstraction of drinking water. In the present study, an integrated analytical and bioanalytical approach was applied to investigate the impact of untreated wastewater effluents from the city of Novi Sad, Serbia, into the River Danube. The study was based on three on-site large volume solid phase extracted water samples collected upstream and downstream of the untreated wastewater discharge. Chemical screening with liquid chromatography high resolution mass spectrometry (LC-HRMS) was applied together with a battery of in vitro cell-based bioassays covering important steps of the cellular toxicity pathway to evaluate effects on the activation of metabolism (arylhydrocarbon receptor AhR, peroxisome proliferator activated receptor gamma PPARγ), specific modes of action (estrogen receptor ERα, androgen receptor AR) and adaptive stress responses (oxidative stress, inflammation). Increased effects, significantly changed contamination patterns and higher chemical concentrations were observed downstream of the wastewater discharge. A mass balance approach showed that enhanced endocrine disruption was in good agreement with concentrations of detected hormones, while only a smaller fraction of the effects on xenobiotic metabolism (<1%) and adaptive stress responses (0–12%) could be explained by the detected chemicals. The chemical and effects patterns observed upstream of the discharge point were fairly re-established at about 7 km downstream, demonstrating the enormous dilution capacity of this large river.
dauerhafte UFZ-Verlinkung
König, M., Escher, B.I., Neale, P.A., Krauss, M., Hilscherová, K., Novák, J., Teodorović, I., Schulze, T., Seidensticker, S., Hashmi, M.A.K., Ahlheim, J., Brack, W. (2017):
Impact of untreated wastewater on a major European river evaluated with a combination of in vitro bioassays and chemical analysis
Environ. Pollut. 220 (Part B), 1220 - 1230 10.1016/j.envpol.2016.11.011