Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) LapF and its regulation by Fis affect the cell surface hydrophobicity of Pseudomonas putida
Autor Lahesaare, A.; Ainelo, H.; Teppo, A.; Kivisaar, M.; Heipieper, H.J.; Teras, R.;
Journal / Serie PLOS ONE
Erscheinungsjahr 2016
Department UBT;
Band/Volume 11
Heft 11
Sprache englisch;
POF III (gesamt) T41;
UFZ Querschnittsthemen RU3;
Abstract The ability of bacteria to regulate cell surface hydrophobicity is important for the adaptation to different environmental conditions. The hydrophobicity of cell surface can be determined by several factors, including outer membrane and surface proteins. In this study, we report that an adhesin LapF influences cell surface hydrophobicity of Pseudomonas putida. Cells lacking LapF are less hydrophobic than wild-type cells in stationary growth phase. Moreover, the overexpression of the global regulator Fis decreases surface hydrophobicity by repressing the expression of lapF. Flow cytometry analysis revealed that bacteria producing LapF are more viable when confronted with methanol (a hydrophilic compound) but are more susceptible to 1- octanol (a hydrophobic compound). Thus, these results revealed that LapF is the hydrophobicity factor for the cell surface of P. putida.
ID 17979
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17979
Lahesaare, A., Ainelo, H., Teppo, A., Kivisaar, M., Heipieper, H.J., Teras, R. (2016):
LapF and its regulation by Fis affect the cell surface hydrophobicity of Pseudomonas putida
PLOS One 11 (11), e0166078