Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1021/acs.est.6b04325
Titel (primär) Toxicokinetics of polar chemicals in zebrafish embryo (Danio rerio): Influence of physicochemical properties and of niological processes
Autor Brox, S.; Seiwert, B.; Küster, E. ORCID logo ; Reemtsma, T.
Quelle Environmental Science & Technology
Erscheinungsjahr 2016
Department BIOTOX; ANA
Band/Volume 50
Heft 18
Seite von 10264
Seite bis 10272
Sprache englisch
UFZ Querschnittsthemen RU3;
Abstract The time-resolved uptake of 17 nonionic and ionic polar compounds (logD ≤ 2) with a diversity of functional groups into zebrafish embryos (ZFE) was studied over 96 h of exposure. Among them were pharmaceuticals, pesticides and plant active ingredients. Uptake rates for the diffusion controlled passive uptake through the ZFE membrane ranged from 0.02 to 24 h–1 for the nonionic compounds and were slower for ionic compounds (<0.008–0.08 h–1). The study compounds did not enrich much in the ZFE (median bioconcentration factor of 1, max. 7). Biotransformation significantly influenced the internal concentration of some of the test compounds over time (benzocaine, phenacetin, metribuzin, phenytoin, thiacloprid, valproic acid). For benzocaine, valproic acid and phenacetin several transformation products (TPs) were observed by LC-MS already at early life-stages (before 28 hpf); for benzocaine the TPs comprised >90% of the initial amount taken up into the ZFE. For six compounds internal concentrations remained very low (rel. int. conc. < 0.2). Besides biotransformation (sulfamethoxazole), poor membrane permeability (cimetidine, colchicine) and also affinity to efflux transporters (atropine and chloramphenicol) are the likely reasons for these low internal concentrations. This study outlines that the uptake of polar compounds into ZFE is influenced by their physicochemical properties. However, biological processes, biotransformation and, likely, efflux can strongly affect the internal concentrations already in early developmental stages of the ZFE. This should be considered in future toxicokinetic modeling. The evaluation of the toxicity of chemicals by ZFE requires toxicokinetic studies of the test compounds and their TPs to increase comparability to effects in fish.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17957
Brox, S., Seiwert, B., Küster, E., Reemtsma, T. (2016):
Toxicokinetics of polar chemicals in zebrafish embryo (Danio rerio): Influence of physicochemical properties and of niological processes
Environ. Sci. Technol. 50 (18), 10264 - 10272 10.1021/acs.est.6b04325