Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.jbiotec.2016.06.022 |
| Titel (primär) | Δ9-Tetrahydrocannabinolic acid synthase: The application of a plant secondary metabolite enzyme in biocatalytic chemical synthesis |
| Autor | Lange, K.; Schmid, A.; Julsing, M.K. |
| Quelle | Journal of Biotechnology |
| Erscheinungsjahr | 2016 |
| Department | SOMA |
| Band/Volume | 233 |
| Seite von | 42 |
| Seite bis | 48 |
| Sprache | englisch |
| Keywords | Cannabinoids; Natural product synthesis; Biocatalysis; Two-liquid phase setup; Oxidase |
| UFZ Querschnittsthemen | RU4; |
| Abstract | Delta(9)-Tetrahydrocannabinolic acid synthase (THCAS) from the secondary metabolism of Cannabis sativa L. catalyzes the oxidative formation of an intramolecular C C bond in cannabigerolic acid (CBGA) to synthesize Delta(9)-tetrahydrocannabinolic acid (THCA), which is the direct precursor of Delta(9)-tetrahydrocannabinel (Delta(9)-THC). Aiming on a biotechnological production of cannabinoids, we investigated the potential of the heterologously produced plant oxidase in a cell-free system on preparative scale. THCAS was characterized in an aqueous/organic two-liquid phase setup in order to solubilize the hydrophobic substrate and to allow in situ product removal. Compared to the single phase aqueous setup the specific activity decreased by a factor of approximately 2 pointing to a substrate limitation of CBGA in the two-liquid phase system. However, the specific activity remained stable for at least 3 h illustrating the benefit of the two-liquid phase setup. In a repeated-batch setup, THCAS showed only a minor loss of specific activity in the third batch pointing to a high intrinsic stability and high solvent tolerance of the enzyme. Maximal space-time-yields of 0.121 gL(-1) h(-1) were reached proving the two-liquid phase concept suitable for biotechnological production of cannabinoids. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17826 |
| Lange, K., Schmid, A., Julsing, M.K. (2016): Δ9-Tetrahydrocannabinolic acid synthase: The application of a plant secondary metabolite enzyme in biocatalytic chemical synthesis J. Biotechnol. 233 , 42 - 48 10.1016/j.jbiotec.2016.06.022 |
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