Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1002/eji.201646297 |
| Titel (primär) | High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition |
| Autor | Zimmermann, J.; Hübschmann, T.; Schattenberg, F.; Schumann, J.; Durek, P.; Riedel, R.; Friedrich, M.; Glauben, R.; Siegmund, B.; Radbruch, A.; Müller, S.; Chang, H.-D. |
| Quelle | European Journal of Immunology |
| Erscheinungsjahr | 2016 |
| Department | UMB |
| Band/Volume | 46 |
| Heft | 5 |
| Seite von | 1300 |
| Seite bis | 1303 |
| Sprache | englisch |
| Keywords | Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis |
| UFZ Querschnittsthemen | RU3; |
| Abstract | Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17808 |
| Zimmermann, J., Hübschmann, T., Schattenberg, F., Schumann, J., Durek, P., Riedel, R., Friedrich, M., Glauben, R., Siegmund, B., Radbruch, A., Müller, S., Chang, H.-D. (2016): High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition Eur. J. Immunol. 46 (5), 1300 - 1303 10.1002/eji.201646297 |
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