Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Autor Zimmermann, J.; Hübschmann, T.; Schattenberg, F.; Schumann, J.; Durek, P.; Riedel, R.; Friedrich, M.; Glauben, R.; Siegmund, B.; Radbruch, A.; Müller, S.; Chang, H.-D.;
Journal / Serie European Journal of Immunology
Erscheinungsjahr 2016
Department UMB;
Band/Volume 46
Heft 5
Sprache englisch;
POF III (gesamt) T41;
Keywords Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis
UFZ Querschnittsthemen RU3;
Abstract Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.
ID 17808
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17808
Zimmermann, J., Hübschmann, T., Schattenberg, F., Schumann, J., Durek, P., Riedel, R., Friedrich, M., Glauben, R., Siegmund, B., Radbruch, A., Müller, S., Chang, H.-D. (2016):
High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition
Eur. J. Immunol. 46 (5), 1300 - 1303