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Titel (primär) Reinoculation elucidates mechanisms of bacterial community assembly in soil and reveals undetected microbes
Autor Francioli, D.; Schulz, E.; Purahong, W.; Buscot, F.; Reitz, T.;
Journal / Serie Biology and Fertility of Soils
Erscheinungsjahr 2016
Department BOOEK; iDiv;
Band/Volume 52
Heft 8
Sprache englisch;
POF III (gesamt) T11;
Keywords Reinoculation of sterilized soil;Nutrient-dependent bacterial community dynamics;Niche and neutral assemblage processes;B-ARISA fingerprinting
UFZ Querschnittsthemen RU1
Abstract Soils harbor a huge diversity of microorganisms that participate in various biogeochemical cycles and influence soil fertility. Our knowledge of soil microbiota, however, is limited by the complexity and heterogeneity of soil habitats and the huge microbial diversity. In this study, fertilized and unfertilized soils from a long-term fertilization experiment were gamma-sterilized before self- or cross-inoculation with non-sterilized soil aliquots containing their respective microbial communities. Bacterial community dynamics resulting from each reinoculated treatment was monitored over 90 days using B-ARISA. This study design allowed us to assess the respective influences of two driving factors, original community and soil nutrient availability, on microbial community assemblage dynamics. For all treatments, the bacterial communities that evolved in the sterilized soils differed from those in the original soils and displayed dynamic shifts over time. These shifts were particularly illustrated by the appearance of numerous OTUs that were undetectable in the original soils and suggest that community assembly is primarily determined by niche factors. However, stronger community shifts and higher variations among the replicates were observed after reinoculation of nutrient-rich soil, suggesting an increased impact of stochastic processes on community assembly. Our results demonstrate that reinoculation-based approaches are not only valid for detecting a variety of low abundant soil bacteria but also for distinguishing the soil characteristics and ecological rules that shape soil microbial population assemblage.
ID 17768
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17768
Francioli, D., Schulz, E., Purahong, W., Buscot, F., Reitz, T. (2016):
Reinoculation elucidates mechanisms of bacterial community assembly in soil and reveals undetected microbes
Biol. Fert. Soils 52 (8), 1073 - 1083