Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Influence of agglomeration and specific lung lining lipid/protein interaction on short-term inhalation toxicity
Autor Wohlleben, W.; Driessen, M.D.; Raesch, S.; Schaefer, U.F.; Schulze, C.; von Vacano, B.; Vennemann, A.; Wiemann, M.; Ruge, C.A.; Platsch, H.; Mues, S.; Ossig, R.; Tomm, J.M.; Schnekenburger, J.; Kuhlbusch, T.A.J.; Luch, A.; Lehr, C.-M.; Haase, A.;
Journal / Serie Nanotoxicology
Erscheinungsjahr 2016
Department MOLSYB;
Band/Volume 10
Heft 7
Sprache englisch;
POF III (gesamt) F11;
Keywords Lipid corona, lung surfactant, protein corona, silica nanoparticles, surface functionalization
UFZ Querschnittsthemen RU3;
Abstract Lung lining fluid is the first biological barrier nanoparticles (NPs) encounter during inhalation. As previous inhalation studies revealed considerable differences between surface functionalized NPs with respect to deposition and toxicity, our aim was to investigate the influence of lipid and/or protein binding on these processes. Thus, we analyzed a set of surface functionalized NPs including different SiO2 and ZrO2 in pure phospholipids, CuroSurfTM and purified native porcine pulmonary surfactant (nS). Lipid binding was surprisingly low for pure phospholipids and only few NPs attracted a minimal lipid corona. Additional presence of hydrophobic surfactant protein (SP) B in CuroSurfTM promoted lipid binding to NPs functionalized with Amino or PEG residues. The presence of the hydrophilic SP A in nS facilitated lipid binding to all NPs. In line with this the degree of lipid and protein affinities for different surface functionalized SiO2 NPs in nS followed the same order (SiO2 Phosphate ∼ unmodified SiO2 < SiO2 PEG < SiO2 Amino NPs). Agglomeration and biomolecule interaction of NPs in nS was mainly influenced by surface charge and hydrophobicity. Toxicological differences as observed in short-term inhalation studies (STIS) were mainly influenced by the core composition and/or surface reactivity of NPs. However, agglomeration in lipid media and lipid/protein affinity appeared to play a modulatory role on short-term inhalation toxicity. For instance, lipophilic NPs like ZrO2, which are interacting with nS to a higher extent, exhibited a far higher lung burden than their hydrophilic counterparts, which deserves further attention to predict or model effects of respirable NPs.
ID 17703
dauerhafte UFZ-Verlinkung
Wohlleben, W., Driessen, M.D., Raesch, S., Schaefer, U.F., Schulze, C., von Vacano, B., Vennemann, A., Wiemann, M., Ruge, C.A., Platsch, H., Mues, S., Ossig, R., Tomm, J.M., Schnekenburger, J., Kuhlbusch, T.A.J., Luch, A., Lehr, C.-M., Haase, A. (2016):
Influence of agglomeration and specific lung lining lipid/protein interaction on short-term inhalation toxicity
Nanotoxicology 10 (7), 970 - 980