Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Sample enrichment for bioanalytical assessment of disinfected drinking water: concentrating the polar, the volatiles, and the unknowns
Autor Stalter, D.; Peters, L.I.; O’Malley, E.; Tang, J.Y.M.; Revalor, M.; Farré, M.J.; Watson, K.; von Gunten, U.; Escher, B.I.;
Journal / Serie Environmental Science & Technology
Erscheinungsjahr 2016
Department ZELLTOX;
Band/Volume 50
Heft 12
Sprache englisch;
POF III (gesamt) T32;
UFZ Querschnittsthemen RU2;
Abstract Enrichment methods used in sample preparation for the bioanalytical assessment of disinfected drinking water result in the loss of volatile and hydrophilic disinfection byproducts (DBPs) and hence likely tend to underestimate biological effects. We developed and evaluated methods that are compatible with bioassays, for extracting nonvolatile and volatile DBPs from chlorinated and chloraminated drinking water to minimize the loss of analytes. For nonvolatile DBPs, solid-phase extraction (SPE) with TELOS ENV as solid phase performed superior compared to ten other sorbents. SPE yielded >70% recovery of nonpurgeable adsorbable organic halogens (AOX). For volatile DBPs, cryogenic vacuum distillation performed unsatisfactorily. Purge and cold-trap with crushed ice serving as condensation nuclei achieved recoveries of 50–100% for trihalomethanes and haloacetonitriles and approximately 60–90% for purged AOX from tap water. We compared the purgeable versus the nonpurgeable fraction by combining purge-and-trap extraction with SPE. The purgeable DBP fraction enriched with the purge-and-trap method exerted a lower oxidative stress response in mammalian cells than the nonpurgeable DBPs enriched with SPE after purging, while contributions of both fractions to bacterial cytotoxicity was more variable. 37 quantified DBPs explained almost the entire AOX in the purge-and-trap extracts, but <16% in the SPE extracts demonstrating that the nonpurgeable fraction is dominated by unknown DBPs.
ID 17682
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17682
Stalter, D., Peters, L.I., O’Malley, E., Tang, J.Y.M., Revalor, M., Farré, M.J., Watson, K., von Gunten, U., Escher, B.I. (2016):
Sample enrichment for bioanalytical assessment of disinfected drinking water: concentrating the polar, the volatiles, and the unknowns
Environ. Sci. Technol. 50 (12), 6495 - 6505