Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Metabolism of clofibric acid in zebrafish embryos (Danio rerio) as determined by liquid chromatography–high resolution–mass spectrometry
Autor Brox, S.; Seiwert, B.; Haase, N.; Küster, E.; Reemtsma, T.;
Journal / Serie Comparative Biochemistry and Physiology C-Toxicology & Pharmacology
Erscheinungsjahr 2016
Department BIOTOX; ANA; ZELLTOX;
Band/Volume 185–186
Sprache englisch;
POF III (gesamt) T42;
Keywords Analytical chemistry; Drug metabolism; Environmental contaminants; Toxicity; Toxicokinetics; Xenobiotic
UFZ Querschnittsthemen RU3;
Abstract The zebrafish embryo (ZFE) is increasingly used in ecotoxicology research but detailed knowledge of its metabolic potential is still limited. This study focuses on the xenobiotic metabolism of ZFE at different life-stages using the pharmaceutical compound clofibric acid as study compound. Liquid chromatography with quadrupole-time-of-flight mass spectrometry (LC-QToF-MS) is used to detect and to identify the transformation products (TPs). In screening experiments, a total of 18 TPs was detected and structure proposals were elaborated for 17 TPs, formed by phase I and phase II metabolism. Biotransformation of clofibric acid by the ZFE involves conjugation with sulfate or glucuronic acid, and, reported here for the first time, with carnitine, taurine, and aminomethanesulfonic acid. Further yet unknown cyclization products were identified using non-target screening that may represent a new detoxification pathway. Sulfate containing TPs occurred already after 3 h of exposure (7 hpf), and from 48 h of exposure (52 hpf) onwards, all TPs were detected. The detection of these TPs indicates the activity of phase I and phase II enzymes already at early life-stages. Additionally, the excretion of one TP into the exposure medium was observed. The results of this study outline the high metabolic potential of the ZFE with respect to the transformation of xenobiotics. Similarities but also differences to other test systems were observed. Biotransformation of test chemicals in toxicity testing with ZFE may therefore need further consideration.
ID 17531
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17531
Brox, S., Seiwert, B., Haase, N., Küster, E., Reemtsma, T. (2016):
Metabolism of clofibric acid in zebrafish embryos (Danio rerio) as determined by liquid chromatography–high resolution–mass spectrometry
Comp. Biochem. Physiol. C-Toxicol. Pharmacol. 185–186 , 20 - 28