Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.mimet.2009.07.010 |
| Titel (primär) | Sequence Specific Primer Extension RNA Analysis (SeSPERA) for the investigation of substrate utilization of microbial communities |
| Autor | Franchini, A.G.; Nikolausz, M.; Kästner, M. |
| Quelle | Journal of Microbiological Methods |
| Erscheinungsjahr | 2009 |
| Department | UBT; BIOENERGIE |
| Band/Volume | 79 |
| Heft | 1 |
| Seite von | 111 |
| Seite bis | 113 |
| Sprache | englisch |
| Keywords | 16S rRNA; Community structure; Microbial activity; Multiplex detection; Radioisotope probing; Specific primer elongation |
| Abstract | A simple, fast and cost-effective method for the taxon-specific separation of various 16S rRNAs was developed. This primer extension mediated separation of different RNA species was obtained on agarose gels. The method provides an easy way for the investigation of substrate mediated radioisotope incorporation into the RNA. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=175 |
| Franchini, A.G., Nikolausz, M., Kästner, M. (2009): Sequence Specific Primer Extension RNA Analysis (SeSPERA) for the investigation of substrate utilization of microbial communities J. Microbiol. Methods 79 (1), 111 - 113 10.1016/j.mimet.2009.07.010 |
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