Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.chemosphere.2016.02.052 |
| Titel (primär) | Combined chemoassay and mass spectrometric approach to study the reactive potential of electrophiles towards deoxynucleosides as model for DNA |
| Autor | Schmied-Tobies, M.I.H.; Paschke, H.; Reemtsma, T. |
| Quelle | Chemosphere |
| Erscheinungsjahr | 2016 |
| Department | ANA |
| Band/Volume | 151 |
| Seite von | 263 |
| Seite bis | 270 |
| Sprache | englisch |
| Keywords | Risk assessment; High resolution mass spectrometry; Adductomics |
| UFZ Querschnittsthemen | RU3; |
| Abstract | The modification of DNA by adduct formation is a potential molecular initiating event of genotoxicity. A chemoassay was established to study adduct formation of electrophiles with deoxynucleosides. Liquid chromatography-mass spectrometry was used to determine the reactivity of the model electrophiles para-benzoquinone, hydroquinone, and 1,4-naphthoquinone with deoxynucleoside (deoxyadenosine (dA), deoxyguanosine (dG), deoxycytidine (dC) and thymidine (dT)) to detect formation of adducts via constant neutral loss scan of deoxyribose (116 Da), and to elucidate adduct structures using high resolution mass spectrometry. Of the four deoxynucleosides dG was most susceptible, followed by dC and para-benzoquinone was the most reactive electrophile. With this approach five dG and four dC adducts were detected, formed by Michael addition and subsequent condensation. Also oxidation occurred with reactive oxygen species (ROS). Three of the adducts formed by benzoquinone have not been reported before. This chemoassay combined with mass spectrometry offers a way (a) to screen a large number of chemicals for their genotoxic potential, (b) to determine novel adducts that may be searched for in in vitro and in vivo studies and thus (c) to better understand the reaction of electrophiles with nucleobases. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17412 |
| Schmied-Tobies, M.I.H., Paschke, H., Reemtsma, T. (2016): Combined chemoassay and mass spectrometric approach to study the reactive potential of electrophiles towards deoxynucleosides as model for DNA Chemosphere 151 , 263 - 270 10.1016/j.chemosphere.2016.02.052 |
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