Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1080/08927014.2016.1169402 |
Titel (primär) | The biofilm matrix of Campylobacter jejuni determined by fluorescence lectin-binding analysis |
Autor | Turonova, H.; Neu, T.R.; Ulbrich, P.; Pazlarova, J.; Tresse, O. |
Quelle | Biofouling |
Erscheinungsjahr | 2016 |
Department | FLOEK |
Band/Volume | 32 |
Heft | 5 |
Seite von | 597 |
Seite bis | 6008 |
Sprache | englisch |
Keywords | Campylobacter jejuni; biofilm matrix; CLSM; FLBA; amyloids |
UFZ Querschnittsthemen | RU2; |
Abstract | Campylobacter jejuni is responsible for the most common bacterial foodborne gastroenteritis. Despite its fastidious growth, it can survive harsh conditions through biofilm formation. In this work, fluorescence lectin-binding analysis was used to determine the glycoconjugates present in the biofilm matrix of two well-described strains. Screening of 72 lectins revealed strain-specific patterns with six lectins interacting with the biofilm matrix of both strains. The most common sugar moiety contained galactose and N-acetylgalactosamine. Several lectins interacted with N-acetylglucosamine and sialic acid, probably originated from the capsular polysaccharides, lipooligosaccharides and N-glycans of C. jejuni. In addition, glycoconjugates containing mannose and fucose were detected within the biofilm, which have not previously been found in the C. jejuni envelope. Detection of thioflavin T and curcumin highlighted the presence of amyloids in the cell envelope without association with specific cell appendages. The lectins ECA, GS-I, HMA and LEA constitute a reliable cocktail to detect the biofilm matrix of C. jejuni. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17350 |
Turonova, H., Neu, T.R., Ulbrich, P., Pazlarova, J., Tresse, O. (2016): The biofilm matrix of Campylobacter jejuni determined by fluorescence lectin-binding analysis Biofouling 32 (5), 597 - 6008 10.1080/08927014.2016.1169402 |