Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) The biofilm matrix of Campylobacter jejuni determined by fluorescence lectin-binding analysis
Autor Turonova, H.; Neu, T.R.; Ulbrich, P.; Pazlarova, J.; Tresse, O.;
Journal / Serie Biofouling
Erscheinungsjahr 2016
Department FLOEK;
Band/Volume 32
Heft 5
Sprache englisch;
POF III (gesamt) T32;
Keywords Campylobacter jejuni; biofilm matrix; CLSM; FLBA; amyloids
UFZ Querschnittsthemen RU2;
Abstract

Campylobacter jejuni is responsible for the most common bacterial foodborne gastroenteritis. Despite its fastidious growth, it can survive harsh conditions through biofilm formation. In this work, fluorescence lectin-binding analysis was used to determine the glycoconjugates present in the biofilm matrix of two well-described strains. Screening of 72 lectins revealed strain-specific patterns with six lectins interacting with the biofilm matrix of both strains. The most common sugar moiety contained galactose and N-acetylgalactosamine. Several lectins interacted with N-acetylglucosamine and sialic acid, probably originated from the capsular polysaccharides, lipooligosaccharides and N-glycans of C. jejuni. In addition, glycoconjugates containing mannose and fucose were detected within the biofilm, which have not previously been found in the C. jejuni envelope. Detection of thioflavin T and curcumin highlighted the presence of amyloids in the cell envelope without association with specific cell appendages. The lectins ECA, GS-I, HMA and LEA constitute a reliable cocktail to detect the biofilm matrix of C. jejuni.

ID 17350
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17350
Turonova, H., Neu, T.R., Ulbrich, P., Pazlarova, J., Tresse, O. (2016):
The biofilm matrix of Campylobacter jejuni determined by fluorescence lectin-binding analysis
Biofouling 32 (5), 597 - 6008