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Titel (primär) Pulsed 13C2-acetate protein-SIP unveils Epsilonproteobacteria as dominant acetate utilizers in a sulfate-reducing microbial community mineralizing benzene
Autor Starke, R.; Keller, A.; Jehmlich, N.; Vogt, C.; Richnow, H.H.; Kleinsteuber, S.; von Bergen, M.; Seifert, J.;
Journal / Serie Microbial Ecology
Erscheinungsjahr 2016
Department ISOBIO; UMB; MOLSYB;
Band/Volume 71
Heft 4
Sprache englisch;
POF III (gesamt) T41;
Keywords Anaerobic benzene degradation; Metaproteomics; Carbon flow
UFZ Querschnittsthemen ProVIS; RU3;
Abstract In a benzene-degrading and sulfate-reducing syntrophic consortium, a clostridium affiliated to the genus Pelotomaculum was previously described to ferment benzene while various sulfate-reducing Deltaproteobacteria and a member of the Epsilonproteobacteria were supposed to utilize acetate and hydrogen as key metabolites derived from benzene fermentation. However, the acetate utilization network within this community was not yet unveiled. In this study, we performed a pulsed 13C2-acetate protein stable isotope probing (protein-SIP) approach continuously spiking low amounts of acetate (10 μM per day) in addition to the ongoing mineralization of unlabeled benzene. Metaproteomics revealed high abundances of Clostridiales followed by Syntrophobacterales, Desulfobacterales, Desulfuromonadales, Desulfovibrionales, Archaeoglobales, and Campylobacterales. Pulsed acetate protein-SIP results indicated that members of the Campylobacterales, the Syntrophobacterales, the Archaeoglobales, the Clostridiales, and the Desulfobacterales were linked to acetate utilization in descending abundance. The Campylobacterales revealed the fastest and highest 13C incorporation. Previous experiments suggested that the activity of the Campylobacterales was not essential for anaerobic benzene degradation in the investigated community. However, these organisms were consistently detected in various hydrocarbon-degrading and sulfate-reducing consortia enriched from the same aquifer. Here, we demonstrate that this member of the Campylobacterales is the dominant acetate utilizer in the benzene-degrading microbial consortium.
ID 17325
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17325
Starke, R., Keller, A., Jehmlich, N., Vogt, C., Richnow, H.H., Kleinsteuber, S., von Bergen, M., Seifert, J. (2016):
Pulsed 13C2-acetate protein-SIP unveils Epsilonproteobacteria as dominant acetate utilizers in a sulfate-reducing microbial community mineralizing benzene
Microb. Ecol. 71 (4), 901 - 911