Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1021/acs.est.5b04819
Volltext Akzeptiertes Manuskript
Titel (primär) Hydrogen isotope fractionation as a tool to identify aerobic and anaerobic PAH biodegradation
Autor Kümmel, S.; Starke, R.; Chen, G.; Musat, F.; Richnow, H.H.; Vogt, C.
Journal / Serie Environmental Science & Technology
Erscheinungsjahr 2016
Department ISOBIO
Band/Volume 50
Heft 6
Seite von 3091
Seite bis 3100
Sprache englisch
UFZ Querschnittsthemen RU4;
Abstract Aerobic and anaerobic polycyclic aromatic hydrocarbon (PAH) biodegradation was characterized by compound specific stable isotope analysis (CSIA) of the carbon and hydrogen isotope effects of the enzymatic reactions initiating specific degradation pathways, using naphthalene and 2-methylnaphtalene as model compounds. Aerobic activation of naphthalene and 2-methylnaphthalene by Pseudomonas putida NCIB 9816 and Pseudomonas fluorescens ATCC 17483 containing naphthalene dioxygenases was associated with moderate carbon isotope fractionation (εC = −0.8 ± 0.1‰ to −1.6 ± 0.2‰). In contrast, anaerobic activation of naphthalene by a carboxylation-like mechanism by strain NaphS6 was linked to negligible carbon isotope fractionation (εC = −0.2 ± 0.2‰ to −0.4 ± 0.3‰). Notably, anaerobic activation of naphthalene by strain NaphS6 exhibited a normal hydrogen isotope fractionation (εH = −11 ± 2‰ to −47 ± 4‰), whereas an inverse hydrogen isotope fractionation was observed for the aerobic strains (εH = +15 ± 2‰ to +71 ± 6‰). Additionally, isotope fractionation of NaphS6 was determined in an overlaying hydrophobic carrier phase, resulting in more reliable enrichment factors compared to immobilizing the PAHs on the bottle walls without carrier phase. The observed differences especially in hydrogen fractionation might be used to differentiate between aerobic and anaerobic naphthalene and 2-methylnaphthalene biodegradation pathways at PAH-contaminated field sites.
dauerhafte UFZ-Verlinkung
Kümmel, S., Starke, R., Chen, G., Musat, F., Richnow, H.H., Vogt, C. (2016):
Hydrogen isotope fractionation as a tool to identify aerobic and anaerobic PAH biodegradation
Environ. Sci. Technol. 50 (6), 3091 - 3100