Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.anaerobe.2016.03.013
Titel (primär) A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters
Autor Bühligen, F.; Lucas, R.; Nikolausz, M.; Kleinsteuber, S. ORCID logo
Quelle Anaerobe
Erscheinungsjahr 2016
Department UMB
Band/Volume 39
Seite von 114
Seite bis 116
Sprache englisch
Keywords mcrA; Community fingerprinting; BstNI; MwoI; Methanogenic archaea; Biogas
UFZ Querschnittsthemen RU4;
Abstract We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.
Bühligen, F., Lucas, R., Nikolausz, M., Kleinsteuber, S. (2016):
A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters
Anaerobe 39 , 114 - 116 10.1016/j.anaerobe.2016.03.013