Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) U6 snRNA intron insertion occurred multiple times during fungi evolution
Autor Canzler, S.; Stadler, P.F.; Hertel, J.;
Journal / Serie RNA Biology
Erscheinungsjahr 2016
Department MOLSYB;
Band/Volume 13
Heft 2
Sprache englisch;
POF III (gesamt) T42; F11;
Keywords Fungi; homology search; intron; snRNA; snRNA evolution
UFZ Querschnittsthemen RU3;
Abstract U6 small nuclear RNAs are part of the splicing machinery. They exhibit several unique features setting them appart from other snRNAs. Reports of introns in structured non-coding RNAs have been very rare. U6 genes, however, were found to be interrupted by an intron in several Schizosaccharomyces species and in 2 Basidiomycota. We conducted a homology search across 147 currently available fungal genome and identified the U6 genes in all but 2 of them. A detailed comparison of their sequences and predicted secondary structures showed that intron insertion events in the U6 snRNA were much more common in the fungal lineage than previously thought. Their positional distribution across the entire mature snRNA strongly suggests a large number of independent events. All the intron sequences reported here show canonical splice site and branch site motifs indicating that they require the splicesomal pathway for their removal.
ID 17254
dauerhafte UFZ-Verlinkung
Canzler, S., Stadler, P.F., Hertel, J. (2016):
U6 snRNA intron insertion occurred multiple times during fungi evolution
RNA Biol. 13 (2), 119 - 127