<< zurück

Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Metacommunity analysis of amoeboid protists in grassland soils
Autor Fiore-Donno, A.M.; Weinert, J.; Wubet, T.; Bonkowski, M.;
Journal / Serie Scientific Reports
Erscheinungsjahr 2016
Department BOOEK; iDiv;
Band/Volume 6
Sprache englisch;
POF III (gesamt) T11;
Keywords Biodiversity; Grassland ecology; Microbial ecology; Soil microbiology
UFZ Querschnittsthemen RU1
Abstract This study reveals the diversity and distribution of two major ubiquitous groups of soil amoebae, the genus Acanthamoeba and the Myxomycetes (plasmodial slime-moulds) that are rarely, if ever, recovered in environmental sampling studies. We analyzed 150 grassland soil samples from three Biodiversity Exploratories study regions in Germany. We developed specific primers targeting the V2 variable region in the first part of the small subunit of the ribosomal RNA gene for high-throughput pyrotag sequencing. From ca. 1 million reads, applying very stringent filtering and clustering parameters to avoid overestimation of the diversity, we obtained 273 acanthamoebal and 338 myxomycete operational taxonomic units (OTUs, 96% similarity threshold). This number is consistent with the genetic diversity known in the two investigated lineages, but unequalled to date by any environmental sampling study. Only very few OTUs were identical to already known sequences. Strikingly different OTUs assemblages were found between the three German regions (PerMANOVA p.value = 0.001) and even between sites of the same region (multiple-site Simpson-based similarity indices <0.4), showing steep biogeographical gradients.
ID 17077
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17077
Fiore-Donno, A.M., Weinert, J., Wubet, T., Bonkowski, M. (2016):
Metacommunity analysis of amoeboid protists in grassland soils
Sci. Rep. 6 , art. 19068