Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Distribution of Dehalococcoidia in the anaerobic deep water of a remote meromictic crater lake and detection of Dehalococcoidia-derived reductive dehalogenase homologous genes
Autor Biderre-Petit, C.; Dugat-Bony, E.; Mege, M.; Parisot, N.; Adrian, L.; Moné, A.; Denonfoux, J.; Peyretaillade, E.; Debroas, D.; Boucher, D.; Peyret, P.;
Journal / Serie PLOS ONE
Erscheinungsjahr 2016
Department ISOBIO;
Band/Volume 11
Heft 1
Sprache englisch;
POF III (gesamt) T41;
Supplements https://doi.org/10.1371/journal.pone.0145558.s001
https://doi.org/10.1371/journal.pone.0145558.s002
https://doi.org/10.1371/journal.pone.0145558.s003
https://doi.org/10.1371/journal.pone.0145558.s004
https://doi.org/10.1371/journal.pone.0145558.s005
UFZ Querschnittsthemen RU3;
Abstract Here we describe the natural occurrence of bacteria of the class Dehalococcoidia (DEH) and their diversity at different depths in anoxic waters of a remote meromictic lake (Lake Pavin) using 16S rRNA gene amplicon sequencing and quantitative PCR. Detected DEH are phylogenetically diverse and the majority of 16S rRNA sequences have less than 91% similarity to previously isolated DEH 16S rRNA sequences. To predict the metabolic potential of detected DEH subgroups and to assess if they encode genes to transform halogenated compounds, we enriched DEH-affiliated genomic DNA by using a specific-gene capture method and probes against DEH-derived 16S rRNA genes, reductive dehalogenase genes and known insertion sequences. Two reductive dehalogenase homologous sequences were identified from DEH-enriched genomic DNA, and marker genes in the direct vicinity confirm that gene fragments were derived from DEH. The low sequence similarity with known reductive dehalogenase genes suggests yet-unknown catabolic potential in the anoxic zone of Lake Pavin.
ID 17037
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17037
Biderre-Petit, C., Dugat-Bony, E., Mege, M., Parisot, N., Adrian, L., Moné, A., Denonfoux, J., Peyretaillade, E., Debroas, D., Boucher, D., Peyret, P. (2016):
Distribution of Dehalococcoidia in the anaerobic deep water of a remote meromictic crater lake and detection of Dehalococcoidia-derived reductive dehalogenase homologous genes
PLOS One 11 (1), e0145558