Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.envpol.2015.11.005 |
| Titel (primär) | Characterization of phenol and cresol biodegradation by compound-specific stable isotope analysis |
| Autor | Wei, X.; Gilevska, T.; Wetzig, F.; Dorer, C.; Richnow, H.-H.; Vogt, C. |
| Quelle | Environmental Pollution |
| Erscheinungsjahr | 2016 |
| Department | ISOBIO |
| Band/Volume | 210 |
| Seite von | 166 |
| Seite bis | 173 |
| Sprache | englisch |
| Keywords | LC-IRMS; Carbon isotope fractionation; Benzylsuccinate synthase; Monooxygenase; p-Cresol methyl hydroxylase |
| UFZ Querschnittsthemen | RU3; |
| Abstract | Microbial degradation of phenol and cresols can occur under oxic and anoxic conditions by different degradation pathways. One recent technique to take insight into reaction mechanisms is compound-specific isotope analysis (CSIA). While enzymes and reaction mechanisms of several degradation pathways have been characterized in (bio)chemical studies, associated isotope fractionation patterns have been rarely reported, possibly due to constraints in current analytical methods. In this study, carbon enrichment factors and apparent kinetic isotope effects (AKIEc) of the initial steps of different aerobic and anaerobic phenol and cresols degradation pathways were analyzed by isotope ratio mass spectrometry connected with liquid chromatography (LC-IRMS). Significant isotope fractionation was detected for aerobic ring hydroxylation, anoxic side chain hydroxylation, and anoxic fumarate addition, while anoxic carboxylation reactions produced small and inconsistent fractionation. The results suggest that several microbial degradation pathways of phenol and cresols are detectable in the environment by CSIA |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=17016 |
| Wei, X., Gilevska, T., Wetzig, F., Dorer, C., Richnow, H.-H., Vogt, C. (2016): Characterization of phenol and cresol biodegradation by compound-specific stable isotope analysis Environ. Pollut. 210 , 166 - 173 10.1016/j.envpol.2015.11.005 |
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