Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1002/pmic.201400139 |
| Volltext | Shareable Link |
| Titel (primär) | Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation |
| Autor | Pagani, M.; Rockstroh, M.; Schuster, M.; Rossetti, G.; Moro, M.; Crosti, M.; Tomm, J.M. |
| Quelle | Proteomics |
| Erscheinungsjahr | 2015 |
| Department | PROTEOM |
| Band/Volume | 15 |
| Heft | 21 |
| Seite von | 3644 |
| Seite bis | 3647 |
| Sprache | englisch |
| Keywords | Cell biology; Human T-lymphocytes; Immunology; LC-MS/MS; Proteome reference map; Th1-cells |
| UFZ Querschnittsthemen | RU3; |
| Abstract | The differentiation of human CD4+ T cells into T helper cell subtypes and regulatory T cells is crucial to the immune response. Among subtypes, Th1 cells are dominant, representing approximately 50% of all lymphocytes. Thus far, most global proteomic studies have used only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or used gel-based approaches. These studies have shed light on molecular details of certain aspects of the proteome; nevertheless a global analysis of high purity primary naïve and Th1 cells by LC-MS/MS is required to provide a reference dataset for proteome-based T cell subtype characterization. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatic comparison to naïve cells reveals changes in cell metabolism and the ubiquitination pathway upon T cell differentiation. All MS data have been deposited in the ProteomeXchange with identifier PXD001066 (http://proteomecentral.proteomexchange.org/dataset/PXD001066). |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16789 |
| Pagani, M., Rockstroh, M., Schuster, M., Rossetti, G., Moro, M., Crosti, M., Tomm, J.M. (2015): Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation Proteomics 15 (21), 3644 - 3647 10.1002/pmic.201400139 |
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