Details zur Publikation

Kategorie Textpublikation
Referenztyp Buchkapitel
Titel (primär) Flow cytometry and microbial community fingerprinting
Titel (sekundär) Flow cytometry in microbiology: Technology and applications
Autor Müller, S.; Schleinitz, K.
Herausgeber Wilkinson, M.G.
Erscheinungsjahr 2015
Department UMB
Seite von 77
Seite bis 89
Sprache englisch
UFZ Querschnittsthemen RU3;
Abstract Flow cytometry (FCM) can be used to reveal community patterns by analyzing certain cell parameters that reflect microbial community structures. The method is valuable for separating a microbial community into sub-sets of individuals with shared physiological properties, such as proliferation activity and scatter characteristics. Fingerprinting tools elucidating community composition, i.e. describing the phylogenetic affiliation of the single community members can also complement FCM. These techniques are introduced with a short overview. Their applications for microbial community composition and structure analyses will be compared. The chapter then describes how fingerprinting techniques can be applied to characterize the FCM-separated subsets. This chapter does not provide detailed protocols for applying FCM to microbial communities, as these protocols will vary depending on the community and physiological parameters of interest. Instead, this chapter highlights the general methodology and approach and discusses potential future advances in the field.
dauerhafte UFZ-Verlinkung
Müller, S., Schleinitz, K. (2015):
Flow cytometry and microbial community fingerprinting
In: Wilkinson, M.G. (ed.)
Flow cytometry in microbiology: Technology and applications
Caister Academic Press, Norfolk, p. 77 - 89