Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.bios.2015.10.069
Titel (primär) Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring
Autor Reinemann, C.; Freiin von Fritsch, U.; Rudolph, S.; Strehlitz, B.
Quelle Biosensors & Bioelectronics
Erscheinungsjahr 2016
Department UBZ
Band/Volume 77
Seite von 2039
Seite bis 1047
Sprache englisch
Keywords Aptamer; SELEX; Antibiotic; Quinolone; Water monitoring
UFZ Querschnittsthemen RU2;
Abstract Quinolones are antibiotics that are accredited in human and veterinary medicine but are regularly used in high quantities also in industrial livestock farming. Since these compounds are often only incompletely metabolized, significant amounts contaminate the aquatic environment and negatively impact on a variety of different ecosystems. Although there is increasing awareness of problems caused by pharmaceutical pollution, available methods for the detection and elimination of numerous pharmaceutical residues are currently inefficient or expensive. While this also applies to antibiotics that may lead to multi-drug resistance in pathogenic bacteria, aptamer-based technologies potentially offer alternative approaches for sensitive and efficient monitoring of pharmaceutical micropollutants. Using the Capture-SELEX procedure, we here describe the selection of an aptamer pool with enhanced binding qualities for fluoroquinolones, a widely used group of antibiotics in both human and veterinary medicine. The selected aptamers were shown to detect various quinolones with high specificity, while specific binding activities to structurally unrelated drugs were not detectable. The quinolone-specific aptamers bound to ofloxacin, one of the most frequently prescribed fluoroquinolone, with high affinity (KD=0.1–56.9 nM). The functionality of quinolone-specific aptamers in real water samples was demonstrated in local tap water and in effluents of sewage plants. Together, our data suggest that these aptamers may be applicable as molecular receptors in biosensors or as catcher molecules in filter systems for improved monitoring and treatment of polluted water.
dauerhafte UFZ-Verlinkung
Reinemann, C., Freiin von Fritsch, U., Rudolph, S., Strehlitz, B. (2016):
Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring
Biosens. Bioelectron. 77 , 2039 - 1047 10.1016/j.bios.2015.10.069