Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Transformation pathways of the recalcitrant pharmaceutical compound carbamazepine by the white-rot fungus Pleurotus ostreatus: Effects of growth conditions
Autor Golan-Rozen, N.; Seiwert, B.; Riemenschneider, C.; Reemtsma, T.; Chefetz, B.; Hadar, Y.;
Journal / Serie Environmental Science & Technology
Erscheinungsjahr 2015
Department ANA;
Band/Volume 49
Heft 20
Sprache englisch;
POF III (gesamt) T42;
Supplements https://pubs.acs.org/doi/suppl/10.1021/acs.est.5b02222/suppl_file/es5b02222_si_001.pdf
UFZ Querschnittsthemen RU3;
Abstract

The widely used anticonvulsant pharmaceutical carbamazepine is recalcitrant in many environmental niches and thus poses a challenge in wastewater treatment. We followed the decomposition of carbamazepine by the white-rot fungus Pleurotus ostreatus in liquid culture compared to solid-state fermentation on lignocellulosic substrate where different enzymatic systems are active. Carbamazepine metabolites were identified using liquid chromatography–high-resolution mass spectrometry (LC-Q-TOF-MS). In liquid culture, carbamazepine was only transformed to 10,11-epoxy carbamazepine and 10,11-dihydroxy carbamazepine as a dead-end product. During solid-state fermentation, carbamazepine metabolism resulted in the generation of an additional 22 transformation products, some of which are toxic. Under solid-state-fermentation conditions, 10,11-epoxy carbamazepine was further metabolized via acridine and 10,11-dihydroxy carbamazepine pathways. The latter was further metabolized via five subpathways. When 14C-carbonyl-labeled carbamazepine was used as the substrate, 14C-CO2 release amounted to 17.4% of the initial radioactivity after 63 days of incubation. The proposed pathways were validated using metabolites (10,11-epoxy carbamazepine, 10,11-dihydroxy carbamazepine, and acridine) as primary substrates and following their fate at different time points. This work highlights the effect of growth conditions on the transformation pathways of xenobiotics. A better understanding of the fate of pollutants during bioremediation treatments is important for establishment of such technologies.

ID 16755
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16755
Golan-Rozen, N., Seiwert, B., Riemenschneider, C., Reemtsma, T., Chefetz, B., Hadar, Y. (2015):
Transformation pathways of the recalcitrant pharmaceutical compound carbamazepine by the white-rot fungus Pleurotus ostreatus: Effects of growth conditions
Environ. Sci. Technol. 49 (20), 12351 - 12362