Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1002/biot.201500059
Volltext Shareable Link
Titel (primär) Environmental stress speeds up DNA replication in Pseudomonas putida in chemostat cultivations
Autor Lieder, S.; Jahn, M.; Koepff, J.; Müller, S.; Takors, R.
Quelle Biotechnology Journal
Erscheinungsjahr 2016
Department UMB
Band/Volume 11
Heft 1
Seite von 155
Seite bis 163
Sprache englisch
Keywords DNA replication; flow cytometry; chemostat; Pseudomonas putida; environmental stress
UFZ Querschnittsthemen RU4;
Abstract Cellular response to different types of stress is the hallmark of the cell's strategy for survival. How organisms adjust their cell cycle dynamics to compensate for changes in environmental conditions is an important unanswered question in bacterial physiology. A cell using binary fission for reproduction passes through three stages during its cell cycle: a stage from cell birth to initiation of replication, a DNA replication phase and a period of cell division. We present a detailed analysis of durations of cell cycle phases, investigating their dynamics under environmental stress conditions. Applying continuous steady state cultivations (chemostats), the DNA content of a Pseudomonas putida KT2440 population was quantified with flow cytometry at distinct growth rates. Data-driven modeling revealed that under stress conditions, such as oxygen deprivation, solvent exposure and decreased iron availability, DNA replication was accelerated correlated to the severity of the imposed stress (up to 1.9 fold). Cells maintained constant growth rates by balancing the shortened replication phase with extended cell cycle phases before and after replication. Transcriptome data underpin the transcriptional upregulation of crucial genes of the replication machinery. Hence adaption of DNA replication speed appears to be an important strategy to withstand environmental stress.
dauerhafte UFZ-Verlinkung
Lieder, S., Jahn, M., Koepff, J., Müller, S., Takors, R. (2016):
Environmental stress speeds up DNA replication in Pseudomonas putida in chemostat cultivations
Biotechnol. J. 11 (1), 155 - 163 10.1002/biot.201500059