Details zur Publikation
|Titel (primär)||What determines chemical uptake by the zebrafish embryo model?|
|Autor||Luckenbach, T. ; Küster, E. ; Busch, W.; Scholz, S.; Altenburger, R.|
|Journal / Serie||Toxicology Letters|
|Abstract||The zebrafish embryo has become a popular model in toxicology and pharmacology. So far, factors determining toxicokinetics of chemicals are rarely considered. Those, however, are important determinants of the bioactive potential of chemicals. The developing embryo is a highly dynamic system undergoing rapid morphological changes from a single cell to a complex organism with differentiated cells and tissues just within one to two days. We found that a P-glycoprotein homolog from zebrafish, a ABC (ATP binding cassette) transporter keeping a diverse array of compounds out of cells, is expressed and active as toxicant defense already very early in development. Activities of metabolic enzymes such as cytochrome P450 homologs acting on a range of toxicants were found in more advanced stages. Overall, our data show that zebrafish embryos possess toxic defence enzymes that have an effect on the uptake, metabolisation and elimination of chemicals; this needs to be considered for toxicokinetic assessments. Further, our studies reveal two important aspects: 1) Activities of relevant enzymes may depend on a certain degree of cellular differentiation, 2) functional properties of relevant zebrafish enzymes can differ from mammalian homologs.|
|Luckenbach, T., Küster, E., Busch, W., Scholz, S., Altenburger, R. (2015):
What determines chemical uptake by the zebrafish embryo model?
Toxicol. Lett. 238 (2, Suppl.), S54