Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium
Autor Simon, A.; Bindschedler, S.; Job, D.; Wick, L.Y.; Filippidou, S.; Kooli, W.M.; Verrecchia, E.P.; Junier, P.;
Journal / Serie FEMS Microbiology Ecology
Erscheinungsjahr 2015
Department UMB;
Band/Volume 91
Heft 11
Sprache englisch;
POF III (gesamt) T41;
Keywords soil; fungal highways; bacteria; fungi; enrichment; columns
UFZ Querschnittsthemen RU3;
Abstract

Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as the recovery of fungi potentially involved in dispersal, which are both attracted towards a target culture medium. We present the validation and the results of a first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax defluvii, Olivibacter soli, and several species of the genera Stenotrophomonas, Achromobacter, and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods.

ID 16634
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16634
Simon, A., Bindschedler, S., Job, D., Wick, L.Y., Filippidou, S., Kooli, W.M., Verrecchia, E.P., Junier, P. (2015):
Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium
FEMS Microbiol. Ecol. 91 (11), fiv116