Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Comparative analysis of goitrogenic effects of phenylthiourea and methimazole in zebrafish embryos
Autor Fetter, E.; Baldauf, L.; Da Fonte, D.F.; Ortmann, J.; Scholz, S.;
Journal / Serie Reproductive Toxicology
Erscheinungsjahr 2015
Department BIOTOX;
Band/Volume 57
Sprache englisch;
POF III (gesamt) T42;
Keywords Endocrine disruption; Adverse outcome pathways; Danio rerio; Behaviour; Alternatives to animal testing; Thyroxine; Teratogenic effects; Ecotoxicology
UFZ Querschnittsthemen RU3;
Abstract Craniofacial malformations, reduced locomotion and induction of genes encoding for enzymes involved in thyroid hormone synthesis were assessed using methimazole and N-phenylthiourea in zebrafish embryos. Gene expression, the most sensitive endpoint (EC50_MMI = 372–765 μM, EC50_PTU = 7.6–8.6 μM), was analysed in wild-type and in a transgenic strain, tg(tg:mCherry), expressing mCherry fluorescence protein under the control of the thyroglobulin gene. Reduction of locomotion and craniofacial malformations were observed at one or two orders of magnitude above concentrations affecting gene expression, respectively. Both effects could be linked to the malformations caused by reduced thyroxin levels. Our results show that due to the presence of the autoregulatory loop of the hypothalamus–pituitary–thyroid axis, various molecular initiating events of thyroid disruption are amenable for the zebrafish embryo. We propose the tg(tg:mCherry) bioassay as a sensitive tool in medium scale screening of goitrogens, given the minimal effort for sample preparation and analysis of gene expression.
ID 16527
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16527
Fetter, E., Baldauf, L., Da Fonte, D.F., Ortmann, J., Scholz, S. (2015):
Comparative analysis of goitrogenic effects of phenylthiourea and methimazole in zebrafish embryos
Reprod. Toxicol. 57 , 10 - 20