Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1007/s00253-015-6599-9 |
| Volltext | Shareable Link |
| Titel (primär) | Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 |
| Autor | Salamanca, D.; Karande, R.; Schmid, A.; Dobslaw, D. |
| Journal / Serie | Applied Microbiology and Biotechnology |
| Erscheinungsjahr | 2015 |
| Department | SOMA |
| Band/Volume | 99 |
| Heft | 16 |
| Seite von | 6889 |
| Seite bis | 6897 |
| Sprache | englisch |
| Keywords | Cyclohexane; CYP450; monooxygenase; Cyclohexanol; Cycloalkanes, Biodegradation |
| UFZ Querschnittsthemen | MIKAT; RU4 |
| Abstract | Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16440 |
| Salamanca, D., Karande, R., Schmid, A., Dobslaw, D. (2015): Novel cyclohexane monooxygenase from Acidovorax sp. CHX100 Appl. Microbiol. Biotechnol. 99 (16), 6889 - 6897 |
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