Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Stable carbon isotope fractionation during 1,2-dichloropropane-to-propene transformation by an enrichment culture containing Dehalogenimonas strains and a dcpA gene
Autor Martín-González, L.; Hatijah Mortan, S.; Rosell, M.; Parladé, E.; Martínez-Alonso, M.; Gaju, N.; Caminal, G.; Adrian, L.; Marco-Urrea, E.;
Journal / Serie Environmental Science & Technology
Erscheinungsjahr 2015
Department ISOBIO;
Band/Volume 49
Heft 14
Sprache englisch;
POF III (gesamt) T41;
UFZ Querschnittsthemen RU3;
Abstract

A stable enrichment culture derived from Besòs river estuary sediments stoichiometrically dechlorinated 1,2-dichloropropane (1,2-DCP) to propene. Sequential transfers in defined anaerobic medium with the inhibitor bromoethanesulfonate produced a sediment-free culture dechlorinating 1,2-DCP in the absence of methanogenesis. Application of previously published genus-specific primers targeting 16S rRNA gene sequences revealed the presence of a Dehalogenimonas strain, and no amplification was obtained with Dehalococcoides-specific primers. The partial sequence of the 16S rRNA amplicon was 100% identical with Dehalogenimonas alkenigignens strain IP3-3. Also, dcpA, a gene described to encode a corrinoid-containing 1,2-DCP reductive dehalogenase was detected. Resistance of the dehalogenating activity to vancomycin, exclusive conversion of vicinally chlorinated alkanes, and tolerance to short-term oxygen exposure is consistent with the hypothesis that a Dehalogenimonas strain is responsible for 1,2-DCP conversion in the culture. Quantitative PCR showed a positive correlation between the number of Dehalogenimonas 16S rRNA genes copies in the culture and consumption of 1,2-DCP. Compound specific isotope analysis revealed that the Dehalogenimonas-catalyzed carbon isotopic fractionation (εCbulk) of the 1,2-DCP-to-propene reaction was −15.0 ± 0.7‰ under both methanogenic and nonmethanogenic conditions. This study demonstrates that carbon isotope fractionation is a valuable approach for monitoring in situ 1,2-DCP reductive dechlorination by Dehalogenimonas strains.

ID 16361
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16361
Martín-González, L., Hatijah Mortan, S., Rosell, M., Parladé, E., Martínez-Alonso, M., Gaju, N., Caminal, G., Adrian, L., Marco-Urrea, E. (2015):
Stable carbon isotope fractionation during 1,2-dichloropropane-to-propene transformation by an enrichment culture containing Dehalogenimonas strains and a dcpA gene
Environ. Sci. Technol. 49 (14), 8666 - 8674