Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) The oxidized state of the nanocomposite Carbo-Iron® causes no adverse effects on growth, survival and differential gene expression in zebrafish
Autor Weil, M.; Meißner, T.; Busch, W.; Springer, A.; Kühnel, D.; Schulz, R.; Duis, K.;
Journal / Serie Science of the Total Environment
Erscheinungsjahr 2015
Department BIOTOX;
Band/Volume 530-531
Sprache englisch;
POF III (gesamt) T42;
Supplements https://ars.els-cdn.com/content/image/1-s2.0-S0048969715301467-mmc1.docx
Keywords Iron-based nanocomposite; Groundwater remediation; Fish toxicity; Uptake; Excretion
UFZ Querschnittsthemen RU3;
Abstract

For degradation of halogenated chemicals in groundwater Carbo-Iron®, a composite of activated carbon and nano-sized Fe0, was developed (Mackenzie et al., 2012). Potential effects of this nanocomposite on fish were assessed. Beyond the contaminated zone Fe0 can be expected to have oxidized and Carbo-Iron was used in its oxidized form in ecotoxicological tests.

Potential effects of Carbo Iron in zebrafish (Danio rerio) were investigated using a 48 h embryo toxicity test under static conditions, a 96 h acute test with adult fish under semi-static conditions and a 34 d fish early life stage test (FELST) in a flow-through system. Particle diameters in test suspensions were determined via dynamic light scattering (DLS) and ranged from 266 to 497 nm. Particle concentrations were measured weekly in samples from the FELST using a method based on the count rate in DLS. Additionally, uptake of particles into test organisms was investigated using microscopic methods. Furthermore, effects of Carbo-Iron on gene expression were investigated by microarray analysis in zebrafish embryos.

In all tests performed, no significant lethal effects were observed. Furthermore, Carbo-Iron had no significant influence on weight and length of fish as determined in the FELST. In the embryo test and the early life stage test, growth of fungi on the chorion was observed at Carbo-Iron concentrations between 6.3 and 25 mg/L. Fungal growth did not affect survival, hatching success and growth. In the embryo test, no passage of Carbo-Iron particles into the perivitelline space or the embryo was observed. In juvenile and adult fish, Carbo-Iron was detected in the gut at the end of exposure. In juvenile fish exposed to Carbo-Iron for 29 d and subsequently kept for 5 d in control water, Carbo-Iron was no longer detectable in the gut. Global gene expression in zebrafish embryos was not significantly influenced by Carbo-Iron.

ID 16217
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=16217
Weil, M., Meißner, T., Busch, W., Springer, A., Kühnel, D., Schulz, R., Duis, K. (2015):
The oxidized state of the nanocomposite Carbo-Iron® causes no adverse effects on growth, survival and differential gene expression in zebrafish
Sci. Total Environ. 530-531 , 198 - 208